CELLULAR IMMUNE FUNCTION IN AIDS AND CANCER

艾滋病和癌症中的细胞免疫功能

• 批准号: 6100958
• 负责人: G M SHEARER
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6100958
• 关键词: AIDS; AIDS vaccines; HIV infections; Wiskott Aldrich syndrome; cellular immunity; cytokine; disease /disorder proneness /risk; helper T lymphocyte; human subject; humoral immunity; immunogenetics; influenza vaccines; longitudinal human study; pediatric AIDS; prognosis; serotyping; tissue /cell culture; virus DNA; virus antigen; virus load

Immunoregulatory cytokine changes result from HIV infection, with decreased type 1 cytokines that enhance cellular immunity (CI) and increased type 2 cytokines that augment humoral immunity (HI). IL-12 and IL-10 enhance CI and HI, respectively, and are produced by monocytes/macrophages (M/M), important targets of HIV infection. M/M from HIV+ patients produced reduced levels of IL-12 and increased levels of IL-10, as did M/M infected in vitro with HIV. IL-12 p40 and p35 mRNA expression was reduced in advanced but not in asymptomatic patients. Therapeutic trials of pediatric AIDS patients with HIV protease inhibitors suggested that patients who presented with reduced viral loads and increased CD4 counts did not exhibit normalized IL-12 and IL- 10 production. This finding suggests that protease inhibitor-induced changes in CD4 count and viral load is not reflected in improved immune function. M/M from uninfected newborns of HIV-infected mothers do not produce IL-12, in contrast to the M/M of newborns of HIV uninfected mothers that do produce IL-12. HIV-induced cytokine dysregulation may be due to a reduction in signal transducers and activators of transcription (STATs), as T cells from HIV-infected patients as well as T cells infected in vitro with HIV-1 exhibited reduced levels of STAT5, but not of the other known STATs. Seronegative, HIV-exposed, T cell- responsive individuals exhibited a dominant type 1 cytokine profile when stimulated with HIV antigens, whereas HIV-infected individuals showed a dominant type 2 cytokine pattern. CD8+ T cell lines resulting from HLA alloantigen mixed lymphocyte stimulation generated a factor that blocked HIV infection of PHA stimulated autologous and allogeneic targets, and also inhibited CMV infection of human fibroblast cell lines. This alloantigen stimulated CD8 anti-viral factor does not appear to be any of the b-chemokines. The factor inhibited T-tropic, M-tropic, and primary isolates of HIV-1, as well as a chronically- infected cell line. Thus, this allo-stimulated factor is capable in vitro of blocking multiple HIV isolates, as well as one of the opportunistic viruses that is problematic in AIDS. AZT was incorporated into the fetal cord blood leukocytes from a majority of women who received AZT during pregnancy. Based on experience from a murine model, this finding raises the possibility of long-term AZT-induced carcinogenesis in the offspring. AIDS title: Immune Defects in HIV/AIDS.

HIV感染导致免疫调节细胞因子变化， 降低增强细胞免疫（CI）的1型细胞因子， 2型细胞因子增加，增强体液免疫（HI）。IL-12和 IL-10分别增强CI和HI，并且由 单核细胞/巨噬细胞（M/M）是HIV感染的重要靶标。M/M 从HIV+患者中产生的IL-12水平降低， IL-10的表达，与体外感染HIV的M/M一样。IL-12 p40和p35 mRNA 在晚期患者中表达降低，但在无症状患者中不降低。 HIV蛋白酶治疗儿童艾滋病的临床研究 抑制剂表明， 负荷和增加的CD 4计数没有表现出正常化的IL-12和IL-12。 10生产这一发现表明，蛋白酶诱导的 CD 4计数和病毒载量的变化并不反映在改善免疫 功能感染艾滋病毒的母亲未感染的新生儿的M/M不 产生IL-12，与未感染HIV的新生儿M/M相比， 产生IL-12的母亲。HIV诱导的细胞因子失调可能 可能是由于信号转导和激活剂的减少， 转录（STAT），如来自HIV感染患者的T细胞以及 在体外用HIV-1感染的T细胞表现出STAT 5水平降低， 而不是其他已知的STAT。血清阴性，艾滋病毒暴露，T细胞- 当反应个体表现出主导的1型细胞因子谱时， 用HIV抗原刺激，而HIV感染者显示 显性2型细胞因子模式。源自HLA的CD 8 + T细胞系 同种抗原混合淋巴细胞刺激产生一种因子， PHA刺激的自体和同种异体靶标的HIV感染，以及 还抑制CMV感染人成纤维细胞系。这 同种异体抗原刺激的CD 8抗病毒因子似乎没有任何 B-趋化因子。该因子抑制T-嗜性、M-嗜性和 HIV-1的原代分离株，以及慢性感染的细胞系。 因此，这种同种异体刺激因子能够在体外阻断 多种HIV分离株，以及一种机会性病毒， 在艾滋病中是个问题。AZT被掺入胎儿脐带血中 大多数在怀孕期间接受AZT的妇女的白细胞。 根据小鼠模型的经验，这一发现提高了 AZT诱导的子代长期致癌的可能性。 艾滋病标题：艾滋病毒/艾滋病的免疫缺陷。