CELL MEDIATED IMMUNITY TO HAPTEN MODIFIED SYNGENEIC LYMPHOCYTES IN MICE

小鼠中针对半抗原修饰的同源淋巴细胞的细胞介导的免疫

• 批准号: 4691755
• 负责人: G M SHEARER
• 金额: --
• 依托单位: DIVISION OF CANCER BIOLOGY AND DIAGNOSIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4691755
• 关键词: T lymphocyte; autologous transplantation; cell cell interaction; cell mediated cytotoxicity; cell population study; cell type; cellular immunity; clone cells; cross immunity; genetic regulation; genetic strain; haptens; helper T lymphocyte; histocompatibility gene; immune tolerance /unresponsiveness; immunogenetics; immunotoxicity; major histocompatibility complex; membrane activity; monoclonal antibody; nitrobenzene; spleen; surface antigens

Mouse spleen cells were modified with trinitrobenezene sulfonate (TNP), and the TNP-self modified cells were tested in two different ways. First, several monoclonal antibodies (mAbs) specific for class I H-2 antigens were tested for binding to TNP-modified spleen cells. Second, the modified cells were used as stimulator and target cells for in vitro tests for cytotoxic T lymphocyte (CTL) responses to TNP-self. A number of anti-H-2Kk antibodies not mAbs of other specificities exhibited enhanced binding to cells that normally express Kk. Furthermore, these same mAbs bound to H-2b cells modified with TNP. These results parallel the patterns of preferential CTL recognition in H-2k mice and of crossreactive CTL in H-2b mouse strains.

用三硝基苯磺酸盐（TNP）修饰小鼠脾细胞， TNP自身修饰的细胞以两种不同的方式进行测试。 第一、 几种对I类H-2抗原特异的单克隆抗体（mAb）， 测试与TNP修饰的脾细胞的结合。 第二，修改 细胞用作体外试验的刺激细胞和靶细胞， 细胞毒性T淋巴细胞（CTL）对自身TNP的应答。 一些抗H-2Kk 抗体而不是其他特异性的mAb表现出增强的与 正常表达Kk的细胞。 此外，这些相同的mAb结合到H-2b 用TNP修饰的细胞。 这些结果与以下模式相似： H-2k小鼠中的优先CTL识别和H-2b中的交叉反应性CTL 小鼠品系。