CORE--SCID-HU MOUSE

核心--SCID-HU鼠标

• 批准号: 6099422
• 负责人: Grace M Aldrovandi
• 金额: $ 14.28万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6099422
• 关键词: AIDS; SCID mouse; animal breeding; animal care; animal colony; biohazard control; biomedical equipment resource; biomedical facility; embryo /fetus tissue transplantation; germ free condition; human fetus tissue; human immunodeficiency virus; tissue mosaicism

Severe combined immunodeficient (SCID) mice are amenable to transplantation with various human tissues and have proven to be valuable tools with which to study HIV pathogenesis, anti-viral efficacy, gene therapy, immunologic development and opportunistic pathogens. The objective of this proposal is to develop a core facility for the breeding, maintenance and reconstitution of SCID mice for experimental use by investigators of the UAB CFAR. The specific aims of the core reflect this objective and are as follows: 1. To provide high-quality pathogen-free SCID mice to UAB CFAR investigators; 2. To provide specially-designed, centrally located BSL 2/3 animals space so that infectious HIV research can be physically restricted to specific areas; 3. To construct hu-PBL SCID mice and limited numbers of SCID-hu (Thy/Liv) for HIV related research; 4. To provide ongoing training, supervision, and monitoring of investigators and their research staff regarding BSL-2/3 practices required for HIV research; 5. To provide expertise on the construction and manipulation of mouse human chimeras and to provide high cost equipment on a shared basis within the core facility in order to facilitate and broaden the research capabilities of all investigators and to avoid redundancy in equipment purchases; 6. To development new mouse/human chimeric models for HIV related research; 7. To foster the development of HIV/AIDS research by new investigators and to encourage multidisciplinary research by new and established investigators.

严重的联合免疫缺陷（SCID）小鼠可及时 用各种人体组织移植，并被证明是有价值的 研究HIV发病机理，抗病毒功效，基因的工具 治疗，免疫发育和机会病原体。这 该建议的目的是为育种开发核心设施， 维护和重建SCID小鼠以实验用途 UAB CFAR的调查人员。核心的具体目的反映了这一点 客观，如下： 1。向UAB CFAR提供高质量的无病原体SCID小鼠 调查人员； 2。提供专门设计的，中心位置的BSL 2/3动物空间 因此，传染性的艾滋病毒研究可以物理地限于特定 区域； 3。构建hu-pbl scid小鼠和有限数量的scid-hu（你/liv） 用于HIV相关研究； 4。提供正在进行的培训，监督和监视 研究人员及其研究人员涉及BSL-2/3实践 艾滋病毒研究所必需的； 5。提供有关鼠标构建和操纵的专业知识 人类嵌合体并在共享的基础上提供高成本设备 为了促进和扩大研究的核心设施 所有调查人员的功能，并避免设备的冗余 购买； 6。开发新的鼠标/人类嵌合模型，用于HIV相关 研究; 7。促进新研究者和 鼓励新的和已建立的多学科研究 调查人员。