CORE--SCID-HU MOUSE

严重联合免疫缺陷(SCID)小鼠易患 与各种人体组织进行移植，并已被证明是有价值的 研究HIV致病机制、抗病毒疗效、基因的工具 治疗、免疫学发展和机会性病原体。这个 这项提议的目标是发展一个繁殖的核心设施， 实验用SCID小鼠的维持和重建 UAB CFAR的调查人员。核心的具体目标反映了这一点 目标和目标如下： 1.为UAB CFAR提供优质无病原体的SCID小鼠 调查员； 2.提供特别设计、位于中心位置的BSL 2/3动物空间 因此，传染性艾滋病毒的研究可以被物理地限制在特定的 地区； 3.HU-PBL SCID小鼠的构建及数量受限的SCID-HU(Thy/Liv) 用于艾滋病毒相关研究； 4.提供持续的培训、监督和监测 关于BSL-2/3实践的调查人员及其研究人员 艾滋病毒研究所需的； 5.提供鼠标的构造和操作方面的专业知识 人类嵌合体，并在共享的基础上提供高成本的设备 核心设施，以便利和扩大研究 所有调查人员的能力，并避免设备冗余 购买； 6.开发与HIV相关的新型鼠/人嵌合模型 研究； 7.促进新调查人员开展艾滋病毒/艾滋病研究，并 鼓励新的和已建立的研究机构进行多学科研究 调查人员。

Severe combined immunodeficient (SCID) mice are amenable to transplantation with various human tissues and have proven to be valuable tools with which to study HIV pathogenesis, anti-viral efficacy, gene therapy, immunologic development and opportunistic pathogens. The objective of this proposal is to develop a core facility for the breeding, maintenance and reconstitution of SCID mice for experimental use by investigators of the UAB CFAR. The specific aims of the core reflect this objective and are as follows: 1. To provide high-quality pathogen-free SCID mice to UAB CFAR investigators; 2. To provide specially-designed, centrally located BSL 2/3 animals space so that infectious HIV research can be physically restricted to specific areas; 3. To construct hu-PBL SCID mice and limited numbers of SCID-hu (Thy/Liv) for HIV related research; 4. To provide ongoing training, supervision, and monitoring of investigators and their research staff regarding BSL-2/3 practices required for HIV research; 5. To provide expertise on the construction and manipulation of mouse human chimeras and to provide high cost equipment on a shared basis within the core facility in order to facilitate and broaden the research capabilities of all investigators and to avoid redundancy in equipment purchases; 6. To development new mouse/human chimeric models for HIV related research; 7. To foster the development of HIV/AIDS research by new investigators and to encourage multidisciplinary research by new and established investigators.