FTICR MASS SPECTRMETRY RESOURCE FOR BIOLOGICAL RESEARCH

用于生物研究的 FTICR 质谱资源

• 批准号: 6188443
• 负责人: RICHARD D SMITH
• 金额: $ 30万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-22 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6188443
• 关键词: FTICR; MASS; SPECTRMETRY; RESOURCE; BIOLOGICAL

A Mass Spectrometry Research Resource at Pacific Northwest National Laboratory for the development and application of advanced instrumental methods in support of biomedical research is described. The facility's Core technological research efforts will involve the development of instrumental methods based upon electrospray ionization (ESI)-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry for the ultra-sensitive characterization of biopolymers. The extremely high resolution and accurate mass measurements feasible with FTICR for larger biopolymers allows more effective identification and provides more extensive structural information. The Resource will utilize nano-scale separation capabilities based upon capillary electrophoresis and micro-fabricated devices for the manipulation of very small samples. In combination with other Core efforts that will improve the overall sensitivity and quality of FTICR, the Resource will enable measurements that are orders of magnitude more sensitive than feasible with conventional instrumentation. These capabilities will be used, for example, for global measurements of proteomes that circumvent the limitations of present 2-D gel technology, and allow rapid studies of protein expression in complex systems with far greater sensitivity than currently possible. The Resource will exploit new instrumentation already available, including a high-magnetic-field (11.5 tesla) FTICR having two complete spectrometers, as well as 3.5 and 7 tesla FTICRs and other mass spectrometric instrumentation. The Resource will leverage a large investment by DOE, as well substantial on-going support that includes equipment maintenance and a unique "collaboratory" research environment. The Resource will serve to make its advanced capabilities available to regional and national collaborators and users in biological research. Important biomedical collaborative efforts will include uniquely informative measurements of neurochemical processes at the single cell level, studies of large protein-protein and protein-DNA interactions not effectively studied with other methods, and the identification of phosphoproteins and their differential state of phosphorylation in T cells committed to a proliferating or anergic state in response to altered peptide ligands.

描述了太平洋西北国家实验室的质谱研究资源，用于开发和应用先进仪器方法以支持生物医学研究。 该设施的核心技术研究工作将涉及开发基于电喷雾电离（ESI）-傅立叶变换离子回旋共振（FTICR）质谱的仪器方法，用于生物聚合物的超灵敏表征。 FTICR 可对较大的生物聚合物进行极高的分辨率和精确的质量测量，从而实现更有效的识别并提供更广泛的结构信息。 该资源将利用基于毛细管电泳和微型制造装置的纳米级分离能力来处理非常小的样品。 与其他提高 FTICR 整体灵敏度和质量的核心工作相结合，该资源将实现比传统仪器的测量灵敏度高几个数量级的测量。 例如，这些功能将用于蛋白质组的全局测量，从而规避当前二维凝胶技术的局限性，并允许以比目前更高的灵敏度快速研究复杂系统中的蛋白质表达。 该资源将利用现有的新仪器，包括具有两个完整光谱仪的高磁场（11.5 特斯拉）FTICR，以及 3.5 和 7 特斯拉 FTICR 以及其他质谱仪器。 该资源将利用美国能源部的巨额投资以及大量持续支持，包括设备维护和独特的“合作”研究环境。该资源将致力于向生物研究领域的区域和国家合作者及用户提供其先进的功能。 重要的生物医学合作工作将包括在单细胞水平上对神经化学过程进行独特的信息测量，对其他方法无法有效研究的大型蛋白质-蛋白质和蛋白质-DNA相互作用的研究，以及对T细胞中磷蛋白及其磷酸化差异状态的识别，这些T细胞响应于改变的肽配体而处于增殖或无反应状态。