MALARIA CONTROL BY GENETIC MANIPULATION OF VECTORS

本项目提案的广泛目标是开发可构成由病媒冈比亚按蚊传播的撒哈拉以南非洲疟疾遗传控制战略基础的工具。本TDRU项目涉及4个具体项目。项目1的具体目标是开发两种不同的安种系转化技术。Gambiae，一种基于转座子的方法和一种位点特异性重组酶方法，用于将较大的DNA片段引入基因组。项目2的目标是开发能够阻断传播阻断抗体的转基因构建物，以及基于小抗寄生虫肽（如cecropins和megainins）的另一种转基因构建物。项目3的目的是开发和测试基于An的抗寄生虫转基因构建体。冈比亚免疫肽将被输送到血液中。项目4的目标是验证减数分裂驱动系统可以作为驱动基因工程寄生虫耐药基因型（如项目2和项目3中产生的那些）进入野生种群的必要工具的假设。

The broad aim of this Project Proposal is to develop the tools that can form the basis of a genetic control strategy for malaria in sub-Saharan African transmitted by the vector Anopheles gambiae. This TDRU Program Project involves 4 specific projects. The specific aims of Project 1 are the development of two different techniques for germ lines transformation of An. Gambiae, a transposon-based method and a site- specific recombinase method for introducing larger fragments of DNA into the genome. The aim of project 2 is to develop transgene constructs that can block transmission blocking antibody and another based on the small, antiparasitic peptides like cecropins and megainins. The aim of project 3 is to develop and test antiparasite transgene constructs based on An. Gambiae immune peptides that will be delivered to the hemocoel. The goal of Project 4 is to test the hypothesis that a meiotic drive system can be used as the necessary tool for driving a genetically engineered parasite refractory genotype (such as those produced in Projects 2 and 3) into the wild population.