Neural Stem Cells From Umbilical Cord Blood

来自脐带血的神经干细胞

• 批准号: 6405632
• 负责人: JUAN R SANCHEZ-RAMOS
• 金额: $ 10.7万
• 依托单位: CCEL BIO-THERAPIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-28 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6405632
• 关键词: cell differentiation; cell proliferation; cell sorting; cord blood; nerve stem cell; nervous system transplantation; phenotype; stem cells; tissue /cell culture; tissue /cell preparation

DESCRIPTION (provided by applicant): A neural stem cell is a single cell with the ability to proliferate, to self-renew over the lifetime of the organism, to generate a large number of clonally-related progeny and to replace limited numbers of neural cells in the adult brain. Recently, several research teams have reported that stem cells obtained from human bone marrow can be induced to differentiate into cells of neuronal and glial phenotype, both in vitro and in vivo. Preliminary results show that umbilical cord blood is a more abundant, readily accessible source of neural precursors than bone marrow. Through isolation, expansion and differentiation of neural stem cells from cord blood, it may be possible to provide enough neural precursors to treat, through transplantation, a variety of neurological deficits induced by neurodegenerative disease, genetic deficit, or injury. This Phase I research program proposes to isolate and characterize neural precursors from the heterogeneous population of mononuclear cells, which comprise umbilical cord blood. The optimal conditions for isolating and differentiating these cells into mature neural cells will be determined. Finally, it will be shown that the neural precursor cells can produce recovery on tests of motor and cognitive function in an animal model of brain injury. The success of directing stem cells obtained from umbilical cord blood into a neural phenotype has important implications for the therapeutic potential of stem cells in neural transplantation in humans. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述（由申请人提供）：神经干细胞是一种单细胞， 增殖的能力，在生物体的一生中自我更新的能力， 产生大量的克隆相关的后代，并取代有限的 成年人大脑中神经细胞的数量。最近，几个研究团队 已经报道了从人骨髓中获得的干细胞可以被诱导为 分化成神经元和神经胶质表型的细胞，无论是在体外还是在体外， vivo.初步结果显示，脐带血是一种更丰富， 比骨髓更容易获得的神经前体来源。通过 从脐带血中分离、扩增和分化神经干细胞， 可以提供足够的神经前体来治疗， 移植，各种神经功能缺损诱导的 神经退行性疾病、遗传缺陷或损伤。第一阶段研究 该计划提出从神经系统中分离和表征神经前体， 单核细胞异质群体，其包括脐带 血分离和分化这些细胞的最佳条件 成熟的神经细胞将被确定。最后，它将表明， 神经前体细胞可以在运动和认知测试中产生恢复 在脑损伤动物模型中的功能。成功的引导茎 从脐带血中获得的细胞转化为神经表型具有重要意义， 干细胞在神经系统疾病中的治疗潜力 人体移植。 拟议商业应用：不可用