TIME RESOLVED SOLUTION XRAY SCATTERING MATURATION PROCESS OF BACTERIOPHAGE HK97

噬菌体 HK97 的时间分辨溶液 X 射线散射成熟过程

• 批准号: 6437697
• 负责人: HIROTSUGU TSURUTA
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437697
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

We have conducted preliminary time-resolved solution scattering studies on the morphological change of bacteriophage HK97 from Prohead II capsid to Head I capsid, one of the most significant maturation processes of this icosahedral phage. After some trial measurements, we discovered that lowering pH from 8.3 to 4.0 effectively transforms Prohead II to an expanded morphology similar to Head I. Further studies were made during the beam time assigned to the letter of intent submitted by J.E. Johnson later in the year.

我们已经进行了初步的时间分辨溶液散射 噬菌体HK97的形态学研究 II衣壳到头I衣壳，其中一个最重要的成熟 这个二十面体噬菌体的过程。 经过一些试验测量， 我们发现，将pH值从8.3降低到4.0， Prohead II的扩张形态与Head I相似。进一步 在分配给字母的光束时间内进行了研究， J. E.提交的意向书约翰逊在今年晚些时候。