FUNCTIONAL GENOMIC/PROTEOMIC ANALYSIS OF BACTERIAL-HOST

细菌宿主的功能基因组/蛋白质组分析

• 批准号: 6394814
• 负责人: John J. Iandolo
• 金额: $ 190.65万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6394814
• 关键词: bacteria infection mechanism; bacterial genetics; biomedical facility; functional /structural genomics; host organism interaction; protein structure function

The purpose off the Institutional Development Award (IdeA) Program is to foster health-related research and increase the competitiveness of investigators through support of faculty development and enhancement of the research infrastructure of institution located in states with historically low aggregate success rates of grant awards from the NIH (RFA +RR-00- 003). Thus, we propose to establish a Center for Biomedical Research Excellence (COBRE), with the thematic focus of Functional genomic/Proteomic Analysis of Bacterial-Host Interactions. This COBRE will exploit one of the few areas of biomedical research where Oklahoma has been in the forefront in recent years, which is the genome-scale analysis of bacterial pathogenesis. Currently, there are five ongoing NIH- supported pathogen genome sequencing programs conducted within the state, including the soon-to-be-completed genome sequence of Neisseria gonorrhoeae, which was the first pathogen genome sequencing project funded by NIH. We therefore propose to build on this unique opportunity by exploring functional genomic and proteome analysis to examine the pathogenesis of five major human pathogens: 1) Neisseria gonorrhoeae, 2) Borrelia burgdorferi, 3) Escherichia coli 0157:H7, 4) Campylobacter jejuni and 5) Bacillus anthracis. The proposed COBRE will be headed by an established NIH-funded investigator and includes three new investigators and an established investigator who do not have a prior history of NIH R01 support. This is a state-wide initiative that includes the thre major publicly-supported research institutions in the state of Oklahoma: 1) the University of Oklahoma (OU), 2) the Oklahoma University Health Sciences Center (OUHSC), institute located near the OUHSC campus. The project is organized similarly to a typical program project grant, facilities on each campus to support the five participating investigators. This strategy will allow each investigator to sufficiently develop their research program to become competitive for additional NIH R01 support. The proposed core facilities also will provide other investigators in Oklahoma with the technical support necessary to expand their own research programs into the burgeoning area of genome-scale analysis of problems of biomedical interest.

机构发展奖（IdeA）计划的目的是通过支持教师发展和加强位于国家的机构的研究基础设施，促进与健康相关的研究，提高研究人员的竞争力。因此，我们建议建立一个生物医学卓越研究中心（COBRE），主题重点是细菌与宿主相互作用的功能基因组/蛋白质组学分析。这个COBRE将利用俄克拉荷马州近年来处于前沿的少数几个生物医学研究领域之一，即细菌致病机理的基因组规模分析。目前，有五个正在进行的NIH支持的病原体基因组测序计划在该州进行，包括即将完成的淋病奈瑟菌基因组测序，这是NIH资助的第一个病原体基因组测序项目。因此，我们建议通过探索功能基因组和蛋白质组分析来研究五种主要人类病原体的发病机制：1）淋病奈瑟菌，2）伯氏疏螺旋体，3）大肠杆菌0157：H7，4）空肠弯曲菌和5）炭疽杆菌。拟议的COBRE将由NIH资助的研究人员领导，包括三名新研究人员和一名没有NIH R 01支持历史的研究人员。这是一项全州范围的倡议，包括俄克拉荷马州的三个主要公共支持的研究机构：1）俄克拉荷马州大学（俄克拉荷马州），2）俄克拉荷马州大学健康科学中心（OUHSC），位于OUHSC校园附近的研究所。该项目的组织类似于一个典型的计划项目赠款，设施在每个校园，以支持五个参与调查。这一策略将允许每个研究者充分发展他们的研究计划，以获得额外的NIH R 01支持。拟议的核心设施还将为俄克拉荷马州的其他研究人员提供必要的技术支持，以将他们自己的研究计划扩展到新兴的生物医学问题基因组规模分析领域。