FUNCTIONAL GENOMIC/PROTEOMIC ANALYSIS OF BACTERIAL-HOST

机构发展奖（IdeA）计划的目的是通过支持教师发展和加强位于美国国立卫生研究院（RFA +RR-00- 003）总成功率较低的州的机构的研究基础设施，促进与健康相关的研究，提高研究人员的竞争力。因此，我们建议建立一个生物医学研究卓越中心（COBRE），以细菌-宿主相互作用的功能基因组学/蛋白质组学分析为主题。这个COBRE将利用俄克拉荷马州近年来一直处于前沿的生物医学研究的少数领域之一，这是细菌发病机制的基因组规模分析。目前，有五个正在进行的NIH支持的病原体基因组测序项目在该州进行，包括即将完成的淋病奈瑟菌基因组测序，这是NIH资助的第一个病原体基因组测序项目。因此，我们建议利用这一独特的机会，通过功能基因组和蛋白质组学分析来研究五种主要人类病原体的发病机制：1)淋病奈瑟菌，2)伯氏疏螺旋体，3)大肠杆菌0157:H7, 4)空肠弯曲杆菌和5)炭疽芽孢杆菌。拟议的COBRE将由一名已建立的NIH资助的研究者领导，包括三名新的研究者和一名没有NIH R01支持历史的已建立的研究者。这是一项全州范围的倡议，包括俄克拉何马州的三个主要公共支持的研究机构：1)俄克拉何马大学（OU）， 2)俄克拉何马大学健康科学中心（OUHSC），位于俄克拉何马大学校园附近的研究所。该项目的组织方式与典型的项目资助类似，每个校区都有支持五名参与研究人员的设施。这一策略将允许每个研究者充分发展他们的研究项目，以获得额外的NIH R01支持。拟议中的核心设施还将为俄克拉何马州的其他研究人员提供必要的技术支持，以将他们自己的研究项目扩展到生物医学兴趣问题的基因组规模分析这一新兴领域。

The purpose off the Institutional Development Award (IdeA) Program is to foster health-related research and increase the competitiveness of investigators through support of faculty development and enhancement of the research infrastructure of institution located in states with historically low aggregate success rates of grant awards from the NIH (RFA +RR-00- 003). Thus, we propose to establish a Center for Biomedical Research Excellence (COBRE), with the thematic focus of Functional genomic/Proteomic Analysis of Bacterial-Host Interactions. This COBRE will exploit one of the few areas of biomedical research where Oklahoma has been in the forefront in recent years, which is the genome-scale analysis of bacterial pathogenesis. Currently, there are five ongoing NIH- supported pathogen genome sequencing programs conducted within the state, including the soon-to-be-completed genome sequence of Neisseria gonorrhoeae, which was the first pathogen genome sequencing project funded by NIH. We therefore propose to build on this unique opportunity by exploring functional genomic and proteome analysis to examine the pathogenesis of five major human pathogens: 1) Neisseria gonorrhoeae, 2) Borrelia burgdorferi, 3) Escherichia coli 0157:H7, 4) Campylobacter jejuni and 5) Bacillus anthracis. The proposed COBRE will be headed by an established NIH-funded investigator and includes three new investigators and an established investigator who do not have a prior history of NIH R01 support. This is a state-wide initiative that includes the thre major publicly-supported research institutions in the state of Oklahoma: 1) the University of Oklahoma (OU), 2) the Oklahoma University Health Sciences Center (OUHSC), institute located near the OUHSC campus. The project is organized similarly to a typical program project grant, facilities on each campus to support the five participating investigators. This strategy will allow each investigator to sufficiently develop their research program to become competitive for additional NIH R01 support. The proposed core facilities also will provide other investigators in Oklahoma with the technical support necessary to expand their own research programs into the burgeoning area of genome-scale analysis of problems of biomedical interest.