FUNCTIONAL GENOMIC/PROTEOMIC ANALYSIS OF BACTERIAL-HOST

机构发展奖(IDEA)计划的目的是通过支持教师发展和加强机构的研究基础设施，促进与健康相关的研究，并增加研究人员的竞争力。这些机构位于国家卫生研究院(NIH)拨款总成功率历史最低的州(RFA RR-00-003)。因此，我们建议建立一个生物医学研究卓越中心(COBRE)，以细菌-宿主相互作用的功能基因组/蛋白质组分析为主题。这个科布雷将探索俄克拉何马州近年来走在前列的为数不多的生物医学研究领域之一，即细菌发病机制的基因组规模分析。目前，该州正在进行五项由NIH支持的病原体基因组测序计划，其中包括即将完成的淋球菌基因组序列，这是NIH资助的第一个病原体基因组测序项目。因此，我们建议以这一独特的机会为基础，通过探索功能基因组和蛋白质组分析来研究五种主要人类病原体的发病机制：1)淋病奈瑟菌，2)伯氏疏螺旋体，3)大肠杆菌0157：H7，4)空肠弯曲杆菌和5)炭疽杆菌。拟议的COBRE将由一名由NIH资助的老牌调查员领导，包括三名新的调查员和一名没有NIH R01支持历史的老牌调查员。这是一项全州范围的倡议，包括俄克拉荷马州三个主要的公共支持的研究机构：1)俄克拉荷马大学(OU)，2)俄克拉荷马大学健康科学中心(OUHSC)，位于OUHSC校园附近的研究所。该项目的组织方式类似于典型的计划项目拨款，即每个校园内的设施，以支持五名参与的调查人员。这一战略将允许每个研究人员充分发展他们的研究计划，使其在获得额外的NIH R01支持方面具有竞争力。拟议的核心设施还将为俄克拉荷马州的其他研究人员提供必要的技术支持，以扩大他们自己的研究计划，进入对生物医学感兴趣的问题进行基因组规模分析的新兴领域。

The purpose off the Institutional Development Award (IdeA) Program is to foster health-related research and increase the competitiveness of investigators through support of faculty development and enhancement of the research infrastructure of institution located in states with historically low aggregate success rates of grant awards from the NIH (RFA +RR-00- 003). Thus, we propose to establish a Center for Biomedical Research Excellence (COBRE), with the thematic focus of Functional genomic/Proteomic Analysis of Bacterial-Host Interactions. This COBRE will exploit one of the few areas of biomedical research where Oklahoma has been in the forefront in recent years, which is the genome-scale analysis of bacterial pathogenesis. Currently, there are five ongoing NIH- supported pathogen genome sequencing programs conducted within the state, including the soon-to-be-completed genome sequence of Neisseria gonorrhoeae, which was the first pathogen genome sequencing project funded by NIH. We therefore propose to build on this unique opportunity by exploring functional genomic and proteome analysis to examine the pathogenesis of five major human pathogens: 1) Neisseria gonorrhoeae, 2) Borrelia burgdorferi, 3) Escherichia coli 0157:H7, 4) Campylobacter jejuni and 5) Bacillus anthracis. The proposed COBRE will be headed by an established NIH-funded investigator and includes three new investigators and an established investigator who do not have a prior history of NIH R01 support. This is a state-wide initiative that includes the thre major publicly-supported research institutions in the state of Oklahoma: 1) the University of Oklahoma (OU), 2) the Oklahoma University Health Sciences Center (OUHSC), institute located near the OUHSC campus. The project is organized similarly to a typical program project grant, facilities on each campus to support the five participating investigators. This strategy will allow each investigator to sufficiently develop their research program to become competitive for additional NIH R01 support. The proposed core facilities also will provide other investigators in Oklahoma with the technical support necessary to expand their own research programs into the burgeoning area of genome-scale analysis of problems of biomedical interest.