CARBOHYDRATE STRUCTURES OF COBRA VENOM FACTOR GLYCOPROTEIN

眼镜蛇毒因子（CVF）是一种分子量为136 kDa的糖蛋白 它能够通过替代的免疫系统激活补体系统 通路 它含有四到五个N-连接的寡糖链， 分子，这是必不可少的功能。 我们正在研究 眼镜蛇CVF中N-连接寡糖的结构 暹罗。 结合核磁共振的结果 光谱和FAB质谱在我们的实验室与 凝集素亲和层析和外切糖苷酶消化的结果 放射性标记糖肽的实验室得出的结论是 CVF中的寡糖是二触角和三触角的 N-乙酰乳糖胺型结构， 高尔？（1？3）加尔？（1？4）[Fuc？（1？3）]GlcNAc？（1？). 其他结构包括 三触角或四触角N-聚糖以及高甘露糖结构。 一份描述这项工作的手稿正在准备中。

Cobra venom factor (CVF) is a 136 kDa molecular mass glycoprotein that is capable of activating the complement system via the alternate pathway. It contains four to five N-linked oligosaccharide chains per molecule, which are essential for its function. We are studying the structures of the N-linked oligosaccharides of CVF from Naja naja siamensis. The combination of results obtained through NMR spectroscopy and FAB-mass spectrometry in our laboratory with the results of lectin affinity chromatography and exoglycosidase digestion of radiolabeled glycopeptides in Dr. Gowda's lab led to the conclusion that the oligosaccharides from CVF are di- and triantennary N-acetyllactosamine-type structures ending in Gal?(1?3)Gal?(1?4)[Fuc?(1?3)]GlcNAc?(1?). Other structures include tri- or tetraantennary N-glycans as well as high-mannose structures. A manuscript is in preparation describing this work.