MALDI & ESI FT ICR MS INVESTIGATIONS OF SAPONINS FROM QUILLAJA SAPONARIA MOLINA

马尔迪

• 批准号: 6478918
• 负责人: Catherine E. Costello
• 金额: $ 5.36万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6478918
• 关键词: biomedical equipment development; biomedical resource; building /facility design /renovation; carbohydrates; lipids; spectrometry; technology /technique

During the assembly and examination of sets of reference post-source decay (PSD) time-of-flight mass spectral data for glycolipids, oligosaccharides and their derivatives, we noted patterns in the small but nevertheless significant calibration errors. Investigation of the sources of these errors led to detailed review of the software implemented by the manufacturer, which had been based on the theoretical and experimental studies of Hillenkamp et al. during the design and testing of the prototype for the commercial Vision 2000 RTOF instrument. These studies brought to light hidden parameters that were instrument-dependent and also showed that there were some errors in the software coding. A new protocol for PSD spectra calibration has been developed, based on the original studies carried out in M(nster and a clear improvement in the mass assignment accuracy has been achieved. The new procedures are being shared with the manufacturer and with other users of reflectron TOF instruments, especially those in the Vision 2000 series.

在组装和检查参考套件时， 源后衰变（PSD）飞行时间质谱数据， 糖脂、寡糖及其衍生物，我们注意到 微小但却显著的校准误差。 对这些错误来源的调查导致了对 制造商实施的软件，该软件基于 Hillenkamp等人的理论和实验研究， 设计和测试商业Vision 2000的原型 RTOF仪器。 这些研究揭示了隐藏的参数 这些都是依赖于仪器的，同时也表明， 软件编码错误。 一种新的PSD谱分析方法 校准已经开发，根据原来的研究进行 在M（nster）中输出，并在质量分配准确性方面有明显改善 办妥了一批多年 新的程序正在与 制造商和反射TOF仪器的其他用户， 特别是在2000年的愿景系列。