A Laser-Activated Reactor for Biomedical C-14-AMS

用于生物医学 C-14-AMS 的激光激活反应器

• 批准号: 6439741
• 负责人: RUTH E SHEFER
• 金额: $ 19.98万
• 依托单位: NEWTON SCIENTIFIC, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-02 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6439741
• 关键词: biomedical equipment development; carbon; cytodiagnosis; fluid; gas; high performance liquid chromatography; lasers; mass spectrometry; particle accelerators; radionuclides

DESCRIPTION (provided by applicant): The goal of this project is to develop the first high efficiency, continuous system for conversion of liquid-phase samples to gas-phase samples for application in C-14 biomedical accelerator mass spectrometry. Analysis of biological samples using AMS is currently hampered by the inability of the instruments to accept liquid-phase samples directly, and in particular the inability to accept samples eluted from conventional separation systems such as HPLC. The proposed system accomplishes liquid-phase sample analysis through a process of evaporative separation of analytes from solvent followed by catalyzed combustion of sample carbon to carbon dioxide to facilitate transport into the AMS ion source. A unique feature of the system is site-specific heating of samples on a catalyst bed with continuously directed laser irradiation of effect combustion of sample carbon in a highly selective and controlled fashion. The system preserves the resolution of samples deposited on the catalyst bed. This system will be coupled to a compact biomedical AMS developed by NSI to demonstrate, for the first time, continuous detection of labeled molecules from an HPLC using an AMS. It will also be suitable for use with other existing AMS systems. PROPOSED COMMERCIAL APPLICATION: The laser-induced sample conversion (LISC) system will have widespread commercial applications in biomedical AMS research in toxicology, drug development, and associated 14C tracer studies. The cost and size of a combined LISC-AMS system will be compatible with installation at major medical research centers. The LISC system will also have a commercial market as a stand-alone product that will facilitate biomedical applications of any existing AMS system.

描述（由申请人提供）： 本项目的目标是开发出第一台高效、连续 用于将液相样品转化为气相样品的系统 在C-14生物医学加速器质谱中的应用。分析 使用AMS的生物样品目前受到无法 直接接受液相样品的仪器，特别是 不能接受从常规分离系统洗脱的样品 HPLC。该系统实现了液相样品分析 通过从溶剂中蒸发分离分析物的过程， 通过将样品碳催化燃烧成二氧化碳， 进入AMS离子源。该系统的一个独特之处是针对具体地点 用连续定向激光加热催化剂床上的样品 辐射影响样品碳在高选择性和高选择性条件下燃烧， 控制时尚该系统保留了样品沉积的分辨率 在催化剂床上。该系统将耦合到一个紧凑的生物医学AMS 由NSI开发，首次展示了连续检测 使用AMS从HPLC获得标记的分子。它也将适合使用 现有的AMS系统。 拟定商业应用： 激光诱导样品转换（LISC）系统将在毒理学、药物开发和相关的14 C示踪研究中的生物医学AMS研究中具有广泛的商业应用。 LISC-AMS组合系统的成本和尺寸将与主要医学研究中心的安装相兼容。 LISC系统还将作为一个独立的产品拥有商业市场，这将促进任何现有AMS系统的生物医学应用。