CRYSTAL STRUCTURE OF YEAST RNA POLYMERASE II SMALL ANGLE DATA

酵母RNA聚合酶II的晶体结构小角度数据

• 批准号: 6586770
• 负责人: PETER R DAVID
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586770
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; parasitism; proteins; radiography; structural biology; technology /technique

We propose collecting the lowest order reflections of RNA polymerase II, from yeast, by using the small-angle scattering expertise and hardware at SSRL on beam line 4-2. H. Tsuruta, of SSRL, has undertaken some preliminary experiments that suggest that 600 E reflections may be obtainable on beam line 4-2, using vacuum beam paths and Imaging Plates. Since the lowest resolution reflections are very important in phasing large proteins to low resolution, we have undertaken to collect heavy atom clusters in a manner that preserves as much information as possible at low resolution.

我们建议收集RNA的最低阶反射 聚合酶II，从酵母，通过使用小角散射 专业技术和硬件在SSRL的光束线4-2。 H. SSRL的鹤田， 已经进行了一些初步的实验，表明600 E 使用真空束，可以在束线4-2上获得反射 路径和成像板。 由于最低分辨率反射是 在将大蛋白质定相到低分辨率方面非常重要，我们有 收集重原子团簇的方式， 尽可能多的信息在低分辨率。