CRYSTAL STRUCTURE OF YEAST RNA POLYMERASE II

酵母 RNA 聚合酶 II 的晶体结构

• 批准号: 6586504
• 负责人: PETER R DAVID
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586504
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

The proposal covers the collection of high resolution native and heavy atom cluster data for RNA-Polymerase II from yeast. RNA-Polymerase II is responsible for the transcription from DNA to RNA of virtually all of the proteins in eukaryotes. There are now a number of co-crystals, with RNA, DNA, RNA+DNA, amanitin (an inhibitor) and promising heavy atom cluster compounds. Because a single data collection can take 36-40 hours, we have requested a large amount of beam time; however, given the unit cell size and the brightness of the source, the data collections cannot be accomplished in less time. It is expected that as the diffraction to higher resolution improves, more time may be needed, as the data will require two or more sweeps.

该提案涵盖了高分辨率本地和 酵母RNA聚合酶II重原子簇数据。 RNA聚合酶II负责从DNA到RNA的转录 真核生物中几乎所有蛋白质的组成部分。 现在有 与RNA、DNA、RNA+DNA、鹅膏蕈碱（一种抑制剂）共晶体的数量 和有前途的重原子簇化合物。 因为一个数据 收集可能需要36-40小时，我们要求大量的 然而，考虑到单位单元的大小和 数据收集不能在更短的时间内完成。 它 预期随着衍射到更高分辨率的改进， 可能需要更多的时间，因为数据将需要两次或更多次扫描。