CRYSTAL STRUCTURE OF YEAST RNA POLYMERASE II

该提案包括收集高分辨率的原生和 酵母RNA聚合酶II的重原子簇数据。 RNA聚合酶II负责DNA到RNA的转录 真核生物中几乎所有的蛋白质。现在有一种 与RNA、DNA、RNA DNA、Amanitin(一种抑制剂)共结晶的数量 以及前景看好的重原子簇合物。因为单个数据 收集可能需要36-40小时，我们已经请求了大量的 波束时间；然而，给定单位单元大小和 数据收集不可能在更短的时间内完成。它 预计随着衍射到更高分辨率的提高， 可能需要更多时间，因为数据将需要两次或更多扫描。

The proposal covers the collection of high resolution native and heavy atom cluster data for RNA-Polymerase II from yeast. RNA-Polymerase II is responsible for the transcription from DNA to RNA of virtually all of the proteins in eukaryotes. There are now a number of co-crystals, with RNA, DNA, RNA+DNA, amanitin (an inhibitor) and promising heavy atom cluster compounds. Because a single data collection can take 36-40 hours, we have requested a large amount of beam time; however, given the unit cell size and the brightness of the source, the data collections cannot be accomplished in less time. It is expected that as the diffraction to higher resolution improves, more time may be needed, as the data will require two or more sweeps.