LOW RESOLUTION YEAST RNA POLYMERASE DATA COLLECTION

低分辨率酵母 RNA 聚合酶数据收集

• 批准号: 6658489
• 负责人: PETER R DAVID
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658489
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

We propose to collect the low and atomic resolution data from yeast RNA polymerase, which is the critical enzyme in gene transcription and regulation. It is highly homologous to mammalian and human enzymes. We have recently obtained atomic diffraction and several new crystal forms that will provide improved diffraction. We are also preparing co-crystals with DNA, inhibitors and heavy atoms. We can obtain only limited diffraction on a rotating anode with long exposures.

我们建议收集低分辨率数据和原子分辨率数据 酵母RNA聚合酶--基因中的关键酶 转录和调控。它与哺乳动物高度同源 和人体酵素。我们最近获得了原子衍射和 几种新的晶体形态将提供改进的衍射。我们 还在制备与DNA、抑制剂和重原子的共晶。 在旋转的长阳极上只能得到有限的衍射。 曝光。