LOW RESOLUTION DATA COLLECTION OF YEAST RNA POLYMERASE II (POL II)

酵母 RNA 聚合酶 II (POL II) 的低分辨率数据采集

• 批准号: 6658529
• 负责人: PETER R DAVID
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658529
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; genome; proteins; radiography; structural biology

We have recently obtained high resolution three dimensional crystals of Pol II at atomic resolution diffraction. We are now pursuing modified crystals, including a tight binding inhibitor and cocomplexes with DNA and DNA/RNA ternary complexes as simple routes to heavy atom derivatives. We have improved our crystals from an earlier limit of 30 E for unfrozen crystals to better than 3.5 E on our recent run using flash freezing and are attempting to improve the purity of the protein for lower mosaicity. Simultaneously, we are attempting to obtain the lowest resolution reflections for a direct phasing of the protein.

我们最近获得了高分辨率的三维图像 Pol II晶体的原子分辨率衍射。 我们现在 追求改性晶体，包括紧密结合抑制剂， 与DNA和DNA/RNA三元复合物的共复合物作为简单的途径， 重原子衍生物 我们已经改进了我们的水晶， 限制30 E为解冻晶体优于3.5 E对我们最近 使用快速冷冻运行，并试图提高纯度， 降低蛋白质的镶嵌性。 与此同时，我们正试图 获得最低分辨率反射，用于直接定相 蛋白