MAD PHASING OF SELENOMETHIONYL CA2+ FREE MANNOSE BINDING PROTEIN C
MAD PHASING OF SELENOMETHIONYL CA2+ FREE MANNOSE BINDING PROTEIN C
批准号:
6658484
负责人:
William I Weis
金额:
$14.32万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-03-01 至 2003-02-28
中文摘要
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英文摘要
C-type lectins are a family of Ca2+-dependent carbohydrate-binding
proteins that undergo a reversible conformational change that renders
them unable to bind Ca2+, hence carbohydrates, at mildly acid pH. In
order to understand this structural transition, we have crystallized
the Ca2+-free form of the lectin domain of mannose-binding protein C.
We have prepared this protein with selenomethionine substituted for
methionine, and we propose to solve the structure by multiwavelength
anomalous dispersion phasing of the selenomethionyl protein, using the
tuneable 1-5AD beamline to collect the data.
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