BD FACS VANTAGE/DiVa cell sorter

描述（由申请人提供）：申请在佛蒙特大学（UVM）购买新的BD FACS Vantage/DiVa细胞分选仪，以替换使用13年的Coulter Elite流式细胞仪。主要原因是需要高速分选、4路分选和6色分析。尽管进行了所有可能的升级，但现有Elite仪器无法满足这些需求。UVM的流式细胞术设施有13名主要研究者，他们定期使用该设施，其中5名主要用户（各>9%）。所有这些研究人员都有联邦资助（12人获得NIH赠款，1人获得美国农业部资助）以及其他基金赠款。共有23个赠款（19个NIH）支持设施大学。因此，它服务于一个非常广泛的社区，需要满足许多不同的要求。选择BD FACS Vantage/DiVa而不是其他流式细胞仪是因为其具有高速分选的综合能力，具有4路分选选项和数字补偿，这在其他仪器上是不可用的。UVM流式细胞仪设施由一位在流式细胞仪领域拥有13年经验的经验丰富的经理管理。它有一个来自免疫生物学计划的教师顾问，拥有18年的流式细胞术经验，以及一个内部咨询委员会，他们都是流式细胞术的长期用户。该设施对所有UVM调查人员开放，是校园内最繁忙的核心设施之一。距离UVM两小时车程内没有其他流式细胞仪设施，校园内也没有其他类似的细胞分选机可供研究人员使用。因此，获得和维护一台新机器对UVM的众多研究人员的研究至关重要。

DESCRIPTION (provided by applicant): A request is made for the purchase of a new BD FACS Vantage/DiVa cell sorter at The University of Vermont (UVM) to replace the 13-year old Coulter Elite flow cytometer. The main reasons are the need for high-speed sorting, 4-way sorting, and 6 color analysis. None of these needs can be met with the existing Elite instrument despite having made all possible upgrades. The flow cyometry facility at UVM has 13 Principal Investigators who use the facility on a regular basis, with 5 major users (>9% each). All of these investigators have federal funding (12 with NIH grants, 1 with a USDA grant) plus other foundation grants. There are a total of 23 grants (19 NIH) that support the facility university. It is thus serving a very broad community and needs to fulfill many different requirements. The BD FACS Vantage/DiVa was chosen over other flow cytometers because of the combined abilities for high-speed sorting, with 4-way sorting options, and digital compensation, which is collectively not available on other instruments. The UVM flow cytometry facility is run by a highly experienced manager with 13 years of experience in flow cytometry. It has a faculty advisor from the Immunobiology Program with 18 years of flow cytometry experience, as well as an internal advisory committee who are all long-time flow cytometry users. The facility is open to all UVM investigators and is one of the busiest core facilities on campus. There is no other flow cytometry facility within a two-hour drive of UVM, and no other comparable cell sorter on campus is available to investigators. Thus, obtaining and maintaining a new machine is absolutely critical to the research of numerous investigators at UVM.