CORE--CELL TISSUE IMAGING FACILITY

核心——细胞组织成像设备

• 批准号: 6989516
• 负责人: SIMON C WATKINS
• 金额: $ 5.48万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-22 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6989516
• 关键词: bioimaging /biomedical imaging; biomedical facility; cytology; electron microscopy; fluorescence microscopy; fluorescence resonance energy transfer; image processing; neoplasm /cancer; neoplastic cell

Over the last several years, microscopy as a scientific tool has reinvented itself. It has changed from a group of principally descriptive methodologies, to a wide range of primary tools and techniques to investigate the molecular organization of organs, tissues and cells. Advances in microscope and camera design, fluorescent dye technology and the development of fluorescent proteins and multiple other novel imaging methods including 2 photon, FRET, TIRF and luminescence imaging has made the simultaneous resolution and quantitation of multiple concurrent molecular markers for both protein and DNA at a sub-micron resolution a reality. The recognition of the potential utility of these techniques to the rapidly expanding research community here at the University of Pittsburgh Cancer Institute (UPC D led to the formation of a centralized microscope imaging center; the Center for Biologic Imaging (CBI), eleven years ago. Since then the CBI has become an essential resource for most of the research programs within UPCI. The Center, which forms the Cell and Tissue imaging core within this proposal performs microscopic analyses services for members of the UPCI research community, from the traditional to the cutting edge using a full complement of microscope technologies. Since the last review of this facility the UPCI has relocated to the Shadyside campus, this has meant that a satellite facility of the Imaging Core will be established in the new Hiltman research building specifically to supply imaging capabilities to researchers in this building. This demands significant investment in instrumentation and personnel. To offset these costs, we have submitted a proposal to the NCRR to provide instrumentation for this building, however we will use the support from this application specifically to provide support for personnel who will work in this new facility in the Shadyside campus.

在过去的几年里，显微镜作为一种科学工具进行了自我改造。它已经从一组主要的描述性方法，转变为研究器官、组织和细胞的分子组织的广泛的主要工具和技术。显微镜和照相机设计的进步，荧光染料技术和荧光蛋白的发展，以及包括双光子、FRET、TIRF和发光成像在内的多种新的成像方法的发展，使得同时对蛋白质和DNA进行亚微米分辨率的多个分子标记的分辨和定量成为可能。 现实。 11年前，匹兹堡大学癌症研究所(UPC D)认识到这些技术对迅速扩大的研究社区的潜在效用，从而形成了一个集中式显微镜成像中心；生物成像中心(CBI)。从那时起，CBI已经成为UPCI内大多数研究项目的基本资源。该中心是本提案中的细胞和组织成像核心，利用一整套显微镜技术为UPCI研究社区的成员提供从传统到尖端的显微分析服务。自上一次对该设施进行审查以来，UPCI已将该设施迁至Shadyside园区，这意味着成像核心的卫星设施将在新的希尔特曼研究大楼内建立，专门为该大楼的研究人员提供成像能力。这需要在仪器设备和人员方面进行大量投资。为抵销这些成本，我们已向 NCRR将为这座建筑提供仪器设备，但我们将专门使用此应用程序的支持来为将在Shadyside园区的这一新设施工作的人员提供支持。