Imaging Core

成像核心

• 批准号: 10204079
• 负责人: SIMON C WATKINS
• 金额: $ 21.75万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-03 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10204079
• 关键词: Acute Lung Injury; Adult Respiratory Distress Syndrome; Algorithmic Software; Apoptosis; Biological; Biological Process; Biology; Cell Communication; Cell Death; Cell Survival; Cells; Computer Assisted; Confocal Microscopy; Data; Electron Microscopy; Electrons; Epithelial Cells; Experimental Designs; Faculty; Fluorescence Microscopy; Goals; Image; Image Analysis; Imaging Techniques; Immunobiology; Immunosuppression; In Vitro; Individual; Lasers; Light; Lipids; Lung; Measurement; Messenger RNA; Methods; Microscopic; Molecular; Morphology; Myeloid Cells; Necrosis; Optical Methods; Optics; Paper; Pathology; Pathway interactions; Pattern; Pneumonia; Process; Proteins; Research Personnel; Resolution; Risk Factors; Role; Sampling; Secondary to; Services; Structure; Techniques; Technology; Tissue imaging; Tissues; Training; Transmission Electron Microscopy; animal imaging; cell behavior; cell motility; cell type; cellular imaging; chemokine; combinatorial; design; experimental study; in vivo; innate immune function; instrumentation; light microscopy; live cell imaging; programs; protein expression; protein transport

Core C: Abstract The overarching theme of program is to dissect a new concept that in Acute Respiratory Distress Syndrome (ARDS), immunosuppression is a signature manifestation secondary to unique, combinatorial pathways that modulate epithelial and myeloid cell viability and innate immune function. We focus on pneumonia as a conceptual framework to study immunosuppression, as pneumonia is a common cause and risk factor for ARDS. The faculty within the Imaging Core have been collaborating continuously and productively with the PI’s of this program for 23 years in various aspects of immunobiology, lipid biology andairway/pulmonary biology resulting in 37 collaborative papers. The principal roles of the imaging core will be; careful analysis of cellular and molecular processes associated with apoptosis, necrosis, ferroptosis and other cell death mechanisms as needed, characterization and quantification of cell types within tissues, examination of protein expression during cell death, examination of cell-cell interactions in vitro, quantitative analysis of cellular migration in vivo and ex vivo individual cells within tissues, and quantitative measurement of the levels and patterns of expression of chemokines and cell-type markers various samples at all levels of resolution. These studies will employ the full array of current light, and potentially, electron microscopic methods including: single and multicolor fluorescence microscopy, laser confocal microscopy live cell imaging, transmission electron microscopy and computer-aided morphometric analyses . The Center for Biologic Imaging, in which this core service will be performed, is designed for the purpose of providing state of the art microscopic technologies to its users. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this Program Project.

核心C：摘要 程序的总体主题是剖析急性呼吸中的新概念 遇险综合征（ARDS），免疫抑制是继发的签名表现。 独特的组合途径，可调节上皮和髓样细胞活力和先天性 免疫功能。我们专注于肺炎作为研究的概念框架 免疫抑制，因为肺炎是ARDS的常见原因和危险因素。教师 在成像中，核心一直在与PI的PI持续和高效地合作 该计划在免疫生物学，脂质生物学的各个方面已有23年 AndAirway/肺部生物学产生了37篇合作论文。 成像核心的主要作用将是；仔细分析细胞和分子 与细胞凋亡，坏死，铁凋亡和其他细胞死亡机制相关的过程 需要，表征和定量组织内的细胞类型，检查蛋白质 细胞死亡期间的表达，在体外检查细胞细胞相互作用，定量分析 在组织内体内和体内单个细胞中的细胞迁移和定量 测量趋化因子和细胞类型标记的表达水平和模式 各级分辨率的各种样本。这些研究将采用一系列当前 光线和潜在的电子显微镜方法，包括：单色和多色 荧光显微镜，激光共聚焦显微镜活细胞成像，透射电子 显微镜和计算机辅助形态分析。生物成像中心， 该核心服务将执行，目的是为了提供状态 艺术微观技术给用户。执行光学连续体是等效的 该程序项目必不可少的方法包括所有类型的光和电子显微镜。