Imaging Core

成像核心

• 批准号: 8643336
• 负责人: SIMON C WATKINS
• 金额: $ 20.23万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-03 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8643336
• 关键词: Acute Lung Injury; Adult Respiratory Distress Syndrome; Algorithmic Software; Anabolism; Animals; Autophagocytosis; Bacterial Infections; Biology; Cardiolipins; Cells; Clinical; Computer software; Critical Illness; Data; Electron Microscopy; Electrons; Experimental Designs; Failure; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Free Radicals; Generations; Genus Hippocampus; Goals; Health; Histologic; Housing; Image; Image Analysis; Immune; Immune System Diseases; In Vitro; Individual; Inflammation; Injury; Life; Light; Longevity; Lung; Measurement; Measures; Mediator of activation protein; Membrane; Messenger RNA; Methodology; Microscopic; Microscopy; Mitochondria; Optics; Oxygen; Oxygen Consumption; Pathology; Patients; Pneumonia; Process; Proteins; Publications; Research; Research Personnel; Role; Scanning; Scanning Transmission Electron Microscopy Procedures; Signal Transduction; Structure; Techniques; Technology; Testing; Tissues; Toxin; Training; Transmission Electron Microscopy; Universities; cell fixing; cellular imaging; computerized data processing; design; extracellular; in vivo; instrumentation; light microscopy; lung injury; medical specialties; multi-photon; novel; pathogen; programs; ratiometric; research study; trafficking

The Imaging core (Core D) is comprised of a centralized cutting-edge microscopy facility, absolutely essential to the research goals of Projects in this P01 application and housed within the Center for Biologic Imaging (CBI) (www.cbi.pitt.edu) of the University of Pittsburgh. All investigators have made, and continue to make, heavy use of the CBI for facility-specific imaging methodologies. Evidence of the longevity of this use is seen in co-authored publications between the PI and staff of the Core (Watkins, St. Croix, Stolz) and PI's of the individual projects. The imaging specialties afforded by CBI include all ultrastructural electron microscopy (transmission electron microscopy, scanning¿ electron microscopy, immune-electron SEM and TEM), light and fluorescence microscopy (macro dissecting light and fluorescence, epi-fluorescence, confocal scanning and multi-photon imaging), live cell microscopy (transmitted light and fluorescence) and fluorescence specialties like FRET, FRAP spectral analysis and ratiometric imaging. Also critical to data processing, a wide range of image analysis software and technical assistance is available to program investigators.

成像核心（核心D）由一个集中的尖端显微镜设施组成，对于P01应用程序中的项目研究目标绝对必要，并位于匹兹堡大学生物成像中心（CBI）（www.cbi.pitt.edu）内。所有的调查人员都已经并将继续大量使用CBI进行设施特定的成像方法。在核心（Watkins、St.克鲁瓦、Stolz）的主要研究者和工作人员以及各个项目的主要研究者之间共同撰写的出版物中，可以看到这种使用寿命的证据。CBI提供的成像专业包括所有超微结构电子显微镜（透射电子显微镜，扫描电子显微镜，免疫电子SEM和TEM），光学和荧光显微镜（宏观解剖光和荧光，epi-fluorescence，共聚焦扫描和多光子成像），活细胞显微镜（透射光和荧光）和荧光专业，如FRET，FRAP光谱分析和比率成像。对数据处理也至关重要的是，项目调查人员可以获得各种图像分析软件和技术援助。