Imaging Core

成像核心

• 批准号: 8518925
• 负责人: SIMON C WATKINS
• 金额: $ 2.21万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-05 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8518925
• 关键词: Adoptive Transfer; Animals; Apoptosis; Area; Autoradiography; Biological; Biological Assay; Biological Products; Cell Communication; Cell Death; Cell Line; Cells; Clinical; Collaborations; Colorectal Cancer; Complement; Complementary DNA; Computer Assisted; Confocal Microscopy; Consultations; Data; Documentation; Electron Microscopy; Electrons; Fluorescence; Fluorescence Microscopy; Gene Expression; Generations; Genes; Goals; Hematopoietic; Histology; Human; Image; Image Analysis; Imagery; Imaging technology; Immune; Immune response; Immune system; Immunology; Immunomodulators; In Situ; In Situ Hybridization; In Vitro; Individual; Label; Laboratories; Lasers; Life; Light; Location; Luciferases; Magnetic Resonance Imaging; Malignant Glioma; Malignant Neoplasms; Measurement; Messenger RNA; Methods; Microscopic; Modeling; Molecular; Molecular Profiling; Mus; Natural Killer Cells; Optical Methods; Optical reporter; Optics; Pathology processes; Pattern; Play; Process; Productivity; Proteins; Publications; Radioactive; Reagent; Reporter Genes; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Reverse Transcriptase Polymerase Chain Reaction; Rodent Model; Role; Running; Services; Source; Speed; Staining method; Stains; T-Lymphocyte; Techniques; Technology; Therapeutic; Therapeutic Agents; Time; Tissues; Training; Transgenic Mice; Transgenic Organisms; Transmission Electron Microscopy; Tumor Tissue; Universities; Vaccinia virus; Viral; Viral Genes; base; cRNA Probes; cell killing; cell motility; cell type; cellular imaging; chemokine; cost; data sharing; design; experience; image processing; image visualization; in vivo; insight; instrumentation; light microscopy; mRNA Expression; melanoma; molecular imaging; neoplastic cell; novel strategies; optical imaging; population based; pre-clinical research; programs; protein expression; research study; response; tool; trafficking; tumor; tumor progression

The central goal of this program is to understand the therapeutic utility of novel strategies to stimulate Tumor cell killing by the immune system. The Imaging Core will play multiple diverse roles within this program. In summary, our primary roles will be : careful analysis of cellular and molecular processes associated with apoptosis, characterization and quantification of cell types within tumors, examination of protein expression during cell death, examination of cell-cell interactions in vitro, quantitative analysis of protein expression by individual cells within tissues, and measurement of the levels and patterns of expression of chemokines and cell-type markers in tumors and tumor explants. These studies will employ the full array of current light, and potentially, electron microscopic methods including: single and multicolor fluorescence microscopy, laser confocal microscopy, live cell imaging, transmission electron microscopy and computer-aided morphometric analyses. The Center for Biologic Imaging, in which this core (B1) service will be performed, is designed for the purpose of providing state of the art microscopic technologies to its users. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this Program Project. Within the scope of this project at the light microscopic level these methods include: histology, immuno-histology, live cell and in situ hybridization methods. The Core will be used extensively by all projects. Core B2 will provide in situ analysis services to all projects, including: generation and sequence confirmation of gene-specific cDNA templates for probe synthesis; in situ hybridization with radioactive cRNA probes and autoradiography; combined in situ hybridization and immunohistochemical staining; and image capture, analysis, and documentation for data sharing and publication. These analysis services will provide integrated data on chemokine expression profiles and producer cells in tumor-containing tissues provided by all projects. Core B3, will act to provide whole animal MRI services as needed in each project. In the previous submission this core was very highly regarded as such changes have been kept to a minimum and only reflect continued interaction and productivity between the core and the project Pis RELEVANCE (Seeinstructions):

该计划的中心目标是了解刺激肿瘤的新策略的治疗效用 免疫系统杀死细胞。成像核心将在该计划中发挥多种不同的作用。在 总之，我们的主要作用将是：仔细分析细胞和分子过程相关的 细胞凋亡、肿瘤内细胞类型的表征和定量、蛋白质表达的检查 在细胞死亡过程中，体外细胞-细胞相互作用的检查，蛋白表达的定量分析， 组织内的单个细胞，以及测量趋化因子和 肿瘤和肿瘤外植体中的细胞类型标志物。这些研究将采用当前光的全部阵列， 潜在地，电子显微镜方法包括：单荧光显微镜和双荧光显微镜，激光 共聚焦显微镜、活细胞成像、透射电子显微镜和计算机辅助形态测量 分析。生物成像中心将执行此核心（B1）服务，其设计目的是 其目的是为用户提供最先进的显微技术。它配备了执行一个 光学方法的连续体，包括对该计划至关重要的所有类型的光学和电子显微镜 项目在本项目的范围内，在光显微镜水平上，这些方法包括：组织学， 免疫组织学、活细胞和原位杂交方法。核心将被所有人广泛使用 项目核心B2将为所有项目提供现场分析服务，包括：生成和排序 用于探针合成的基因特异性cDNA模板的确认;与放射性cRNA的原位杂交 探针和放射自显影;原位杂交和免疫组织化学染色;图像 捕获、分析和记录数据，以实现数据共享和发布。这些分析服务将提供 关于含肿瘤组织中趋化因子表达谱和生产细胞的综合数据， 所有项目。核心B3将根据每个项目的需要提供整体动物MRI服务。在 以前提交的这一核心是非常高的评价，因为这种变化一直保持在最低限度， 仅反映核心和项目PI之间的持续互动和生产力 相关性（参见说明）：