Request for Zeiss 710 multiphoton 5Live Duoscan Microscope

描述（由申请人提供）：本申请用于购买多光子/单光子高速共聚焦显微镜。该仪器将被安置在匹兹堡大学医学院生物成像中心（CBI）内。该核心设施的任务是为匹兹堡大学医学院的所有研究小组提供全面的光和电子光学，图像分析和形态测量方法。光学切片显微镜是中心提供的一项重要服务，目前中心使用的共聚焦显微镜是一台3年和2年的奥林巴斯FV1000，一台9年的奥林巴斯fluoview 500，一台类似年龄的徕卡TCS SL和一台6年的蔡司510。我们最近还获得了倒置单光子/多光子奥林巴斯FV1000。这个应用程序的原因是：1：为目前在CBI内不能使用倒置系统研究的系统提供使用直立配置的多光子显微镜；为各种项目提供高速共聚焦成像，包括为开发模型系统（如鱼和老鼠）的血流成像，人类气道培养中的纤毛跳动和脑切片中的钙测量。自17年前CBI开始运营以来，它已成为医学研究界不可或缺的一部分，目前参与了200多个由小灵通资助的医学领域小组的研究项目。以及小灵通与邻近机构其他部门的调查人员以及全国各地的合作者合作支持的项目。这里要求的设备是对CBI用户可用的显微镜装备的重要和基本的补充，它将对匹兹堡大学和其他地方的PHS资助的研究产生根本和重大的影响。

DESCRIPTION (provided by applicant): This request is for funds to purchase a multiphoton capable/single photon high speed confocal microscope. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. Optical sectioning microscopy is an essential service provided by the center, the current core use confocal microscopes within the facility are a 3 yr old and a 2 yr old Olympus FV1000, a 9 year old Olympus fluoview 500, a similarly aged Leica TCS SL and a 6 year old Zeiss 510. We have also recently aquired an inverted single photon/multiphoton Olympus FV1000. The reasons for this application are to: 1: Provide access to multiphoton microscopy using an upright configuration for systems which cannot be studied using the inverted system currently within the CBI 2: Provide access to high speed confocal imaging for a variety projects ranging from imaging blood flow in developing model systems such as fish and mice, cillary beat in human airway cultures and calcium measurements in brain slices Since the CBI commenced operations 17 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 200 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments at neighboring institutions, and with collaborators around the country. The device requested here is an important and fundamental addition to the armamentarium of microscopies available to users of the CBI and it will have fundamental and significant impact on PHS funded research at the University of Pittsburgh and beyond.