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Request for a Leica 3X STED Microscope

索取 Leica 3X STED 显微镜

基本信息

批准号：
9074993
负责人：
SIMON C WATKINS
金额：
$ 123.95万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-15 至 2017-03-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This request is for funds to purchase a Leica Stimulated Emission Depletion (STED) 3X microscope with 3 depletion lines. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. Super-resolution microscopy is an essential service provided by the center. Until last year the improvement in resolution using STED, while significant was limited in commercial systems to improvements in the XY axis with no improvements in Z resolution. However, with the release of a STED solution that is effective in all 3 axis the approach has become much more useful. Furthermore when equipped with 3 depletion lines the method works with many conventional fluorophores allowing facile 3 color super-resolution imaging such that it can be easily deployed in a core environment such as the CBI. The need for this instrument is demonstrated in this application with several diverse NIH supported projects in fields including; Neurobiology, Cell Biology, Developmental Biology, Infectious Disease, cytoskeletal biology, and basic Mitochondrial function. Since the CBI commenced operations 24 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 300 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments and at neighboring institutions. The steering committee of the CBI feel that acquisition of a 3X STED system as an essential addition to the repertoire of instrumentation available to PHS funded users of the Center.

描述（由申请人提供）：此请求旨在提供资金购买具有 3 条耗尽线的徕卡受激发射耗尽 (STED) 3X 显微镜。该仪器将安装在匹兹堡大学医学院的生物成像中心 (CBI) 内。该核心设施的任务是为匹兹堡大学医学院的所有研究小组提供全方位的光和电子光学、图像分析和形态测量方法。超分辨率显微镜是该中心提供的一项重要服务。直到去年，使用 STED 的分辨率改进虽然在商业系统中显着仅限于 XY 轴的改进，但 Z 分辨率没有改进。然而，随着在所有 3 轴上均有效的 STED 解决方案的发布，该方法变得更加有用。此外，当配备 3 条耗尽线时，该方法可与许多传统荧光团一起使用，从而实现轻松的 3 色超分辨率成像，从而可以轻松部署在 CBI 等核心环境中。该应用程序通过 NIH 支持的多个不同领域的项目证明了对该仪器的需求，包括：神经生物学、细胞生物学、发育生物学、传染病、细胞骨架生物学和基本线粒体功能。自 24 年前开始运作以来，CBI 已成为医学研究界不可分割的一部分，目前与医疗领域内 300 多个 PHS 资助的团体一起参与研究项目，并与其他部门和邻近机构的研究人员一起参与 PHS 支持的项目。 CBI 指导委员会认为，购买 3X STED 系统是对 PHS 资助的中心用户可用的仪器设备的重要补充。