Imaging Core

核心C：摘要 该计划的首要主题是剖析一个新的概念，在急性呼吸道疾病 窘迫综合征（ARDS），免疫抑制是继发于 独特的，组合的途径，调节上皮和骨髓细胞的活力和先天性 免疫功能我们把肺炎作为一个概念框架来研究 免疫抑制，因为肺炎是ARDS的常见原因和危险因素。学院 在成像核心内，一直与以下PI进行持续有效的合作： 该计划在免疫生物学，脂质生物学的各个方面已有23年 以及气道/肺生物学，共发表37篇合作论文。 成像核心的主要作用是：仔细分析细胞和分子 与细胞凋亡、坏死、铁凋亡和其他细胞死亡机制相关的过程， 需要，组织内细胞类型的表征和定量，蛋白质检查 细胞死亡过程中的表达，体外细胞-细胞相互作用的检查，定量分析 体内和离体组织内单个细胞的细胞迁移，以及定量 测量趋化因子和细胞类型标志物的表达水平和模式 各种分辨率的各种样品。这些研究将采用当前的各种 光，并可能，电子显微镜方法，包括：单和双 荧光显微镜，激光共聚焦显微镜活细胞成像，透射电子显微镜 显微镜和计算机辅助形态测定分析。生物成像中心（Center for Biologic Imaging） 这一核心服务将被执行，是为了提供状态的目的， 艺术显微技术给它的使用者。它配备了一个连续的光学 方法，包括本计划项目所必需的所有类型的光学和电子显微镜。

Core C: Abstract The overarching theme of program is to dissect a new concept that in Acute Respiratory Distress Syndrome (ARDS), immunosuppression is a signature manifestation secondary to unique, combinatorial pathways that modulate epithelial and myeloid cell viability and innate immune function. We focus on pneumonia as a conceptual framework to study immunosuppression, as pneumonia is a common cause and risk factor for ARDS. The faculty within the Imaging Core have been collaborating continuously and productively with the PI’s of this program for 23 years in various aspects of immunobiology, lipid biology andairway/pulmonary biology resulting in 37 collaborative papers. The principal roles of the imaging core will be; careful analysis of cellular and molecular processes associated with apoptosis, necrosis, ferroptosis and other cell death mechanisms as needed, characterization and quantification of cell types within tissues, examination of protein expression during cell death, examination of cell-cell interactions in vitro, quantitative analysis of cellular migration in vivo and ex vivo individual cells within tissues, and quantitative measurement of the levels and patterns of expression of chemokines and cell-type markers various samples at all levels of resolution. These studies will employ the full array of current light, and potentially, electron microscopic methods including: single and multicolor fluorescence microscopy, laser confocal microscopy live cell imaging, transmission electron microscopy and computer-aided morphometric analyses . The Center for Biologic Imaging, in which this core service will be performed, is designed for the purpose of providing state of the art microscopic technologies to its users. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this Program Project.