Core--Proteomics Facility

西特曼癌症中心(SCC)蛋白质组学核心使用当前和新开发的方法和试剂为SCC成员提供多路蛋白质组分析。这些方法包括用多种荧光染色定量和检测官能团的二维差示凝胶电泳法，用串联质谱仪(LC-MS/MS和MALDI-TOF/-TOF)鉴定蛋白质的方法，以及用差示稳定同位素标记的多维液相色谱方法。SCC蛋白质组学核心正在与SCC生物信息学核心(核心主任Jeffrey Milbrandt)和多重基因分析核心(核心主任Mark Watson)合作，建立一个实验室信息管理系统(LIMS)和一个关系数据库，使SCC研究人员能够访问和查询临床、蛋白质组学和 通过单一的医学信息学门户网站获取mRNA阵列数据。

The Siteman Cancer Center (SCC) Proteomics Core provides multiplexed proteomic analysis to members of the SCC using the current and newly developed methods and reagents. These include two-dimensional differential gel electrophoresis with multiple fluorescent stains to quantify and detect functional groups, protein identification using tandem mass spectrometry (LC-MS/MS and MALDI-TOF/-TOF) and multidimensional liquid chromatography methods with differential stable isotope labeling. The SCC Proteomics Core is collaborating with the SCC Bioinformatics Core (Jeffrey Milbrandt, Core Director) and Multiplexed Gene Analysis Core (Mark Watson, Core Director) to build a laboratory information management system (LIMS) and a relational database to enable SCC investigators to access and query clinical, proteomics and mRNA array data through a single medical informatics portal.