CORE--PROTEOMICS FACILITY

核心——蛋白质组学设施

• 批准号: 8168696
• 负责人: ROBERT REID TOWNSEND
• 金额: $ 15.54万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-10 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168696
• 关键词: Bioinformatics; Cancer Center; Clinical; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Genes; Grant; Institution; Laboratories; Liquid Chromatography; Management Information Systems; Medical Informatics; Messenger RNA; Methods; Proteins; Proteomics; Reagent; Research; Research Personnel; Resources; Source; Stable Isotope Labeling; Staining method; Stains; United States National Institutes of Health; functional group; gel electrophoresis; member; relational database; tandem mass spectrometry; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Siteman Cancer Center (SCC) Proteomics Core provides multiplexed proteomic analysis to members of the SCC using the current and newly developed methods and reagents. These include two-dimensional differential gel electrophoresis with multiple fluorescent stains to quantify and detect functional groups, protein identification using tandem mass spectrometry (LC-MS/MS and MALDI-TOF/-TOF) and multidimensional liquid chromatography methods with differential stable isotope labeling. The SCC Proteomics Core is collaborating with the SCC Bioinformatics Core (Jeffrey Milbrandt, Core Director) and Multiplexed Gene Analysis Core (Mark Watson, Core Director) to build a laboratory information management system (LIMS) and a relational database to enable SCC investigators to access and query clinical, proteomics and mRNA array data through a single medical informatics portal.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Siteman癌症中心（SCC）蛋白质组学核心使用当前和新开发的方法和试剂为SCC成员提供多重蛋白质组学分析。其中包括使用多种荧光染料进行二维差异凝胶电泳以定量和检测功能团、使用串联质谱（LC-MS/MS和MALDI-TOF/-TOF）进行蛋白质鉴定以及具有差异稳定同位素标记的多维液相色谱方法。SCC蛋白质组学核心与SCC生物信息学核心（Jeffrey Milbrandt，核心总监）和多重基因分析核心（Mark沃森，核心总监）合作，建立实验室信息管理系统（LIMS）和关系数据库，使SCC研究人员能够通过单一的医学信息学门户访问和查询临床，蛋白质组学和mRNA阵列数据。