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中文摘要
翻译
这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金, 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 西特曼癌症中心(SCC)蛋白质组学核心使用当前和新开发的方法和试剂为SCC成员提供多路蛋白质组分析。这些方法包括用多种荧光染色定量和检测官能团的二维差示凝胶电泳法,用串联质谱仪(LC-MS/MS和MALDI-TOF/-TOF)鉴定蛋白质的方法,以及用差示稳定同位素标记的多维液相色谱方法。SCC蛋白质组学核心正在与SCC生物信息学核心(核心总监Jeffrey Milbrandt)和多重基因分析核心(核心总监Mark Watson)合作,建立一个实验室信息管理系统(LIMS)和一个关系数据库,使SCC研究人员能够通过单一的医疗信息门户访问和查询临床、蛋白质组学和mRNA阵列数据。
英文摘要
This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Siteman Cancer Center (SCC) Proteomics Core provides multiplexed proteomic analysis to members of the SCC using the current and newly developed methods and reagents. These include two-dimensional differential gel electrophoresis with multiple fluorescent stains to quantify and detect functional groups, protein identification using tandem mass spectrometry (LC-MS/MS and MALDI-TOF/-TOF) and multidimensional liquid chromatography methods with differential stable isotope labeling. The SCC Proteomics Core is collaborating with the SCC Bioinformatics Core (Jeffrey Milbrandt, Core Director) and Multiplexed Gene Analysis Core (Mark Watson, Core Director) to build a laboratory information management system (LIMS) and a relational database to enable SCC investigators to access and query clinical, proteomics and mRNA array data through a single medical informatics portal.
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CORE--PROTEOMICS FACILITY
  • 批准号:
    8361346
  • 项目类别:
  • 资助金额:
    $11.87万
  • 财政年份:
    2011
  • 负责人:
    ROBERT REID TOWNSEND
  • 依托单位:
GROUP MEETING IN PROTEOMICS
  • 批准号:
    8361476
  • 项目类别:
  • 资助金额:
    $4.5万
  • 财政年份:
    2011
  • 负责人:
    ROBERT REID TOWNSEND
  • 依托单位:
MEETINGS AND CONFERENCES
  • 批准号:
    8361477
  • 项目类别:
  • 资助金额:
    $3.04万
  • 财政年份:
    2011
  • 负责人:
    ROBERT REID TOWNSEND
  • 依托单位:
CORE--PROTEOMICS FACILITY
  • 批准号:
    8168696
  • 项目类别:
  • 资助金额:
    $15.54万
  • 财政年份:
    2010
  • 负责人:
    ROBERT REID TOWNSEND
  • 依托单位:
海外基金