PROTEOMICS CORE

蛋白质组学，作为“组学”级联(基因组学、转录组学、 蛋白质组学和代谢组学)预计将对临床实践产生深远影响 未来几十年的胃肠病学和肝脏学(Lazaridis等人，2005年)。在过去的五年里， 蛋白质组学的进展以其速度和敏感性的数量级增长为标志 数据获取正在推动生物信息学努力进行数据解释和整合到“组学”中 输油管道。蛋白质组学的“数据收集”技术现在有了坚实的、定量的基础(冯等 Al.，2008)。整合来自相同高质量人体样本的所有“组学”数据是最基本的一步 用于高维生物学在预防、诊断和治疗G1疾病方面的应用，是 迫在眉睫的挑战。如下所述，在《D.生物信息学和数据集成》一节中 蛋白质组学数据与其他‘组学’数据和临床信息是DDRCC蛋白质组学的主要目标 核心。 自从上一次竞争性更新以来，蛋白质组学核心实验室已经演变为包括 将最先进的技术和软件融入到DDRCC调查人员的新颖、全面的工作流程中，如 详情如下。在CTSA的支持下，核心增加了工作人员科学家来提供DDRCC 调查人员在设计和解释统计数据合理且成本效益高的综合体方面的专业知识和协助 临床蛋白质组学实验。芯子开创了样品处理和分析的新方法 使得蛋白质组学和功能基因组学数据可以从相同的样本中产生 取自炎症性肠病患者的组织(见初步结果和Rader等。 2008年)。 蛋白质组学核心的成本效益扩展到资本密集型要求 蛋白质组学分析。复员方案协调中心的资源不足以支持一个独立的单位。随着不断增加 为了满足临床样本分析的能力需求，蛋白质组学核心发起并监督了 提供全天候高级质谱学分析的内部质谱学联盟 临床样本中的蛋白质、多肽和代谢物(见图17)。蛋白质组学核心作为一个 中央机构核心，也得到了国家卫生研究院、国家卫生中心 研究资源(UL1-RR024992(Kenneth Polonsky，Pl)‘和P4r-RR000954(Michael Gix)ss，Pi)， 国家癌症研究所(P30 CA91842，Tim Eberlein，PI)，W.M.Keck基金会，Siteman癌症 中心，以及其他机构资源。通过订阅这一中央设施并支持 进行蛋白质组学实验所需的高素质工作人员，DDRCC可以为其成员提供 折扣价仅包括用品和试剂的价格。

Proteomics, as one of the evolving disciplines of the "omics" cascade (genomics, transcriptomics, proteomics and metabolomics) is projected to have a profound impact on the clinical practice of gastroenterology and hepatology in the coming decades (Lazaridis et al., 2005). Within the past five years, the advances in proteomics have been hallmarked by orders of magnitude increases in speed and sensitivity of data acquisition that is driving bioinformatics efforts for data interpretation and integration into the "omics" pipeline. The "data gathering" technologies for proteomics now have a firm, quantitative foundation (Feng et al., 2008). The integration of all "omics" data from the same high quality human samples is a fundamental step for the application of high-dimensional biology to preventing, diagnosing and treating Gl disorders, and is one of the imminent challenges. As discussed below under 'D. Bioinformatics and Data Integration' the assembly of proteomics data with other 'omics' data and clinical information is a major goal of the DDRCC Proteomics Core. Since the last competitive renewal, the Proteomics Core Laboratory has evolved to incorporate the most advanced technologies and software into novel, comprehensive workflows for DDRCC investigators, as detailed below. With the support of the CTSA, the Core has added staff scientists to provide DDRCC investigators the expertise and assistance to design and interpret statistically sound and cost-effective complex clinical proteomics experiments. The Core has pioneered new methods of sample processing and analysis such that proteomics and functional genomics data can be generated from the same samples that have been procured from tissues of patients with inflammatory bowel diseases (see Preliminary Results and Rader etal., 2008). The cost effectiveness of the Proteomics Core extends to the capital-intense requirements of proteomics analyses. The DDRCC resources are insufficient to support an independent unit. With increasing capacity demands for the analysis of clinical samples, the Proteomics Core has initiated and supervises an intramural mass spectrometry consortium that provides 24/7 advanced mass spectrometric analysis of proteins, peptides and metabolites in clinical samples (see Figure 17). The Proteomics Core serves as a central institutional core and is also supported by the National Institutes of Health, National Centerfor Research Resources (UL1-RR024992 (Kenneth Polonsky, Pl)'and P4r-RR000954 (Michael Gix)ss, PI), National Cancer Institute (P30 CA91842, Tim Eberlein, PI), the W. M. Keck Foundation, the Siteman Cancer Center, and other institutional resources. By subscribing to this central facility and supporting the salaries of the highly qualified staff required to carry out proteomics experiments, the DDRCC can offer its members services at a discounted price that covers only the price of supplies and reagents.