CORE--PROTEOMICS FACILITY

核心——蛋白质组学设施

• 批准号: 8361346
• 负责人: ROBERT REID TOWNSEND
• 金额: $ 11.87万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361346
• 关键词: Bioinformatics; Cancer Center; Clinical; Data; Funding; Genes; Grant; Laboratories; Liquid Chromatography; Management Information Systems; Mass Spectrum Analysis; Medical Informatics; Messenger RNA; Methods; National Center for Research Resources; Principal Investigator; Proteins; Proteomics; Reagent; Research; Research Infrastructure; Research Personnel; Resources; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stable Isotope Labeling; Staining method; Stains; United States National Institutes of Health; biomedical resource; cost; functional group; gel electrophoresis; member; relational database; tandem mass spectrometry; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Siteman Cancer Center (SCC) Proteomics Core provides multiplexed proteomic analysis to members of the SCC using the current and newly developed methods and reagents. These include two-dimensional differential gel electrophoresis with multiple fluorescent stains to quantify and detect functional groups, protein identification using tandem mass spectrometry (LC-MS/MS and MALDI-TOF/-TOF) and multidimensional liquid chromatography methods with differential stable isotope labeling. The SCC Proteomics Core is collaborating with the SCC Bioinformatics Core (Jeffrey Milbrandt, Core Director) and Multiplexed Gene Analysis Core (Mark Watson, Core Director) to build a laboratory information management system (LIMS) and a relational database to enable SCC investigators to access and query clinical, proteomics and mRNA array data through a single medical informatics portal.

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