PHOTON MIGRATION OF LOCALLY ADVANCED BREAST CANCER IMPACT OF NEOADJUVANT THERAPY

描述(由申请人提供)： 激光微束和医学计划(LAMMP)是美国国立卫生研究院生物医学技术资源中心，致力于激光和光学在生物学和医学中的使用。LAMP位于贝克曼激光研究所(BLI)内，这是加州大学欧文分校的一个跨学科生物医学研究、教学和临床设施。BLI在管理上是加州大学欧文分校医学院外科的一个部门。总体资源目标是促进建立一个平衡的中心，开展技术研究和开发、协作/服务研究和培训/传播方面的活动。在LAMP的第五次续签申请中，我们强调我们独特的能力，通过快速将基础科学和技术发现从“台式计算机转移到床边”来促进“转化式”研究。这是通过将最先进的光学技术与我们在细胞和组织工程、动物模型和人体研究方面的内部设施相结合来实现的。LAMP提供微束和显微镜技术(MMT)，用于活细胞和组织的光学操作和功能成像，以及医学翻译技术(MTT)，用于监测、治疗和成像临床前动物模型和人类受试者。总共提出了6个新的技术研究和开发项目，这些项目将导致建造5个新的专用仪器。MMT和MTR技术通过一套共同的光学技术、计算模型、数据可视化方法和生物医学应用程序连接在一起。它们结合在一起，能够以可缩放的分辨率和从微米到厘米的深度灵敏度来表征和成像组织中的结构和生化成分。这允许有选择地询问组织的基本成分：分子、细胞、细胞外基质和血管系统。在整个赠款周期中，我们将把这些新兴的方法应用于生物学模型和临床问题，以表征和量化内在光学信号的精确结构和功能来源。我们的长期目标是推动这些技术的发展，使它们成为广泛可用的，使解决生物学和医学中根本重要问题的方法成为可能。

DESCRIPTION (provided by applicant): The Laser Microbeam and Medical Program (LAMMP) is a NIH Biomedical Technology Resource Center dedicated to the use of lasers and optics in biology and medicine. LAMMP is located within the Beckman Laser Institute (BLI), an interdisciplinary biomedical research, teaching, and clinical facility at the University of California, Irvine. The BLI functions administratively as a division within the Department of Surgery in the Medical School at UC Irvine. Overall resource objectives are to promote a well-balanced Center with activities in technological research and development, collaborative/service research, and training/dissemination. In this fifth renewal application of LAMMP, we emphasize our unique capabilities to facilitate "translational" research by rapidly moving basic science and technology discoveries from "benchtop to bedside". This is accomplished by combining state of the art optical technologies with our in-house facilities in cellular and tissue engineering, animal models, and human subjects. LAMMP provides both Microbeam and Microscopy Technologies (MMT) for optical manipulation and functional imaging of living cells and tissues, and Medical Translational Technologies (MTT) for monitoring, treating, and imaging pre-clinical animal models and human subjects. A total of six new technology research and development projects are proposed that will result in the construction of 5 new, dedicated instruments. MMT and MTr technologies am linked together by a common set of optical technologies, computational models, data visualization methods, and biomedical applications. Combined, they are capable of characterizing and imaging structure and biochemical composition in tissues with scalable resolution and depth sensitivity ranging from microns to centimeters. This allows selective interrogation of the essential components of tissue: molecules, cells, extracellular matrix, and vasculature. Throughout the grant cycle, we will apply these emerging methods to biological models and clinical problems in order to characterize and quantify the precise structural and functional origins of intrinsic optical signals. Our long-term goal is to advance these technologies so they become widely-available, enabling methods for solving fundamentally important problems in biology and medicine.