The evolution of Membrane Attack Complex / Perforin-like proteins in development and immunity

膜攻击复合物/穿孔素样蛋白在发育和免疫中的进化

• 批准号: DP140102771
• 负责人: Prof James Whisstock
• 金额: $ 37.74万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP140102771
• 关键词: evolution; Membrane; Attack; Complex; Perforin

Membrane Attack Complex/Perforin-like (MACPF) proteins commonly play key roles in vertebrate immunity, however some family members play critical but poorly understood developmental roles. It has been shown that the sole Drosophila MACPF protein, Torsolike, plays both developmental and immune roles. Here the aim to unravel how Torsolike functions in these dual roles by using a novel genetic screening approach to identify genes needed for its function. The project also uses comparative studies to probe alternative functions of Torsolike in a second insect, the honeybee. This data will be pivotal for understanding how members of this family have evolved into proteins that are separately involved in immune defense and in development in higher vertebrates.

膜攻击复合体/穿孔素样（MACPF）蛋白通常在脊椎动物免疫中起关键作用，然而一些家族成员在发育中起着关键的作用，但人们对其知之甚少。研究表明，果蝇唯一的MACPF蛋白Torsolike同时具有发育和免疫作用。这里的目的是通过使用一种新的遗传筛选方法来鉴定其功能所需的基因，揭示Torsolike在这些双重角色中的功能。该项目还使用比较研究来探索另一种昆虫——蜜蜂——Torsolike的替代功能。这些数据对于理解这个家族的成员如何进化成单独参与免疫防御和高等脊椎动物发育的蛋白质是至关重要的。