COBRE: LOUISVILLE RES FOUND INC: CORE B: MICROARRAY FACILITY

COBRE：LOUISVILLE RES FOUND INC：核心 B：微阵列设施

• 批准号: 7171225
• 负责人: Wolfgang Zacharias
• 金额: $ 9.68万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7171225
• 关键词: biomedical facility; drug discovery /isolation; neoplasm /cancer chemotherapy; structural biology

DESCRIPTION (provided by applicant): The University of Louisville Molecular Targets Center of Biomedical Research Excellence application (MTCOBRE) is focused on the identification of novel molecular targets for cancer therapy using the techniques of modern structural biology. The two major objectives of this proposal are to ensure the success of junior investigators who are members of the Molecular Targets Program at the University of Louisville and to discover new approaches to the therapy of cancer. The Specific Aims of the Molecular Targets COBRE application are to: 1. Foster successful independent research careers for each of the five principal investigators leading to independent R01 grants. 2. Further build our existing core facilities which will enhance the research capability of the five principal investigators and the other members of the program. 3. Continue to develop our Molecular Targets Program as a cohesive research program which will promote collaborative research projects and increase funding opportunities. 4. Develop an internationally and nationally recognized program which will attract the very best graduate students, postdoctoral fellows and additional faculty. 5. Most importantly, make discoveries leading to new and more effective treatments for patients with cancer. The five related projects of this MT-COBRE application will address a broad spectrum of molecular targets. The specific problems being investigated are: 1) Characterization and manipulation of an inducible isoform of 6-phosphofructo-2-kinase (iPFK-2), a major controller of glucose metabolism in malignant cells. 2) Development and testing of small molecular antagonists of monocyte/macrophage migration inhibitory factor (MIF) recently found to play an important role in carcinogenesis. 3) Elucidation of the signaling mechanisms involved in the activation-dependent lipid signaling enzyme, sphingosine kinase, and its product, sphingosine-l-phosphate, in control of cell proliferation, survival and migration. 4) Use of triplex forming oligonucleotides to target Bleomycin to the Her2/neu gene. 5) A crystallographer/biologist is being recruited to study molecular interactions important in the TRAF signaling pathway. The overarching theme of this program is the use of structural biology to develop novel strategies for therapy of neoplastic disease. We believe that these five projects, along with those of the other members of the program, represent a coherent approach to the application of modern structural biology to translational cancer research.

描述（由申请人提供）： 路易斯维尔大学生物医学研究卓越中心（MTCOBRE）专注于使用现代结构生物学技术鉴定癌症治疗的新型分子靶标。该提案的两个主要目标是确保路易斯维尔大学分子靶点计划成员的初级研究人员的成功，并发现癌症治疗的新方法。Molecular Targets COBRE应用的具体目标是： 1.为五位主要研究者中的每一位培养成功的独立研究事业，从而获得独立的R 01赠款。 2.进一步建设我们现有的核心设施，这将提高五个主要研究者和项目其他成员的研究能力。 3.继续发展我们的分子目标计划作为一个有凝聚力的研究计划，这将促进合作研究项目，并增加资助机会。 4.制定一个国际和国家认可的计划，这将吸引最好的研究生，博士后研究员和其他教师。 5.最重要的是，为癌症患者找到新的更有效的治疗方法。 MT-COBRE应用的五个相关项目将涉及广泛的分子靶点。正在研究的具体问题是：1）6-磷酸果糖-2-激酶（iPFK-2），在恶性细胞中的葡萄糖代谢的主要控制器的诱导型异构体的表征和操作。2)最近发现单核细胞/巨噬细胞移动抑制因子（MIF）的小分子拮抗剂的开发和测试在癌症发生中起重要作用。3)阐明激活依赖性脂质信号传导酶鞘氨醇激酶及其产物鞘氨醇-l-磷酸在控制细胞增殖、存活和迁移中涉及的信号传导机制。4)三链体形成寡核苷酸将博来霉素靶向Her 2/neu基因的用途。5)正在招募一名晶体学家/生物学家来研究TRAF信号通路中重要的分子相互作用。该计划的首要主题是使用结构生物学来开发肿瘤疾病治疗的新策略。我们相信，这五个项目，沿着与该计划的其他成员，代表了一个连贯的方法，现代结构生物学的转化癌症研究的应用。