COBRE: LOUISVILLE RES FOUND INC: CORE B: MICROARRAY FACILITY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 微序列阵列核心设施位于Baxter II生物医学研究大楼，距离布朗癌症中心一个街区。该设施包含一台Affymetrix基因芯片仪器，可供所有MT-Cobre成员使用。开发该设施的组织阵列组件的计划也在进行中。微序列阵列设施由沃尔夫冈·扎卡里亚斯博士领导，在过去四年半的时间里全面投入运作。它包括一名全职工作人员Sabine Waigel，他在样品处理、杂交和数据分析方面提供技术援助。微序列阵列核心与病理部的激光捕获显微镜设备和J.G.布朗癌症中心的已建立的肿瘤库有着密切的关系。这使得制备具有已知病理特征的样本和适当的对照样本成为可能。扎卡里亚斯博士仍然可以在实验设计和数据分析方面提供帮助。 新的高分辨率Affymetrix扫描仪3000升级于2005年3月购买并安装，现在允许所有Cobre研究人员使用最新一代高密度全基因组v2.0阵列，用于人类或小鼠。我们继续在校园内组织和举办软件/数据分析研讨会，以培训微阵列技术用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microsequence Array Core Facility is located in the Baxter II Biomedical Research Building, one block from the Brown Cancer Center. This facility contains an Affymetrix Genechip instrument which is available to all MT-COBRE members. Plans are underway for the development of a tissue array component of this facility as well. The Microsequence Array Facility is under the direction of Dr. Wolfgang Zacharias and has been fully operational for the past four and one-half years. It contains a full-time staff member, Sabine Waigel, who provides technical assistance in sample processing, hybridization, and data analysis. The Microsequence Array Core has a close relationship with the Laser Capture Microscopy facility in the Department of Pathology and an established Tumor Bank in the J.G. Brown Cancer Center. This allows the preparation of samples with known pathologic characteristics and appropriate control specimens. Dr. Zacharias remains available for assistance with experimental design and data analysis. The new high-resolution Affymetrix Scanner 3000 upgrade, purchased and installed in March 2005, now allows all COBRE investigators to use the newest generation of high-density whole genome v2.0 arrays for human or mouse. We continue to organize and hold software/data analysis workshops on campus to train microarray technology users.