Microarray Core

项目总结(见说明)： 在路易斯维尔大学，通过UofL James Graham Brown癌症中心(JGBCC)的投资，用于基于高密度微阵列的基因表达和单核苷酸多态(SNP)基因分型分析的仪器、人员和软件资源已经到位。这样的全球图谱可以在一次节省时间的标准化实验中从细胞群体或组织材料中获得数万个基因或SNP基因座。对生物或病理材料上的全球基因表达模式的比较分析将提供有关细胞和/或疾病过程中涉及的基因产物和/或突变的关键信息。 微阵列核心的目标是为研究人员提供这种高度先进的技术以及足够的培训和研究应用机会。微阵列设施的具体目标是向JGBCC调查人员提供以下服务： 1.为基于微阵列的基因表达和基因分型分析提供优质服务； 2.不断将最新的阵列产品和分析工具纳入所提供的服务菜单； 3.就微阵列技术的应用、优点和局限性对用户进行教育和培训； 4.协助用户进行实验设计、样品处理和数据分析； 5.为应用微阵列技术的项目开发、资助申请和稿件准备提供后勤支持。 这里介绍的核心将确保JGBCC研究人员，特别是临床教员和研究小组，能够有效地采用这一新的最先进的技术，使用体外、临床标本或动物模型材料(使用他们的批准方案)来实现他们的研究、临床和研究目标。至关重要的是，作为向他们提供的机构资源的一部分，这些研究人员能够负担得起获得最先进和复杂的分子技术的机会和专家支持。

PROJECT SUMMARY (See instructions): At the University of Louisville, the instrument, personnel, and software resources for high-density microarray based gene expression and single nucleotide polymorphism (SNP) genotyping analyses are in place through an investment of the UofL James Graham Brown Cancer Center (JGBCC). Such global profiles can be obtained for tens of thousands of genes or SNP loci from cell populations or tissue material within one time saving and standardized experiment. Comparative analysis of global gene expression patterns on biological or pathologic material will provide crucial information on gene products and/or mutations which are involved in a cellular and/or disease process. The objective of the Microarray Core is to offer this highly advanced technology as well as sufficient opportunities for training and research applications for investigators. The Specific Aims of the Microarray Facility are to offer the following services to JGBCC investigators: 1. To provide high-quality services for microarray-based gene expression and genotyping analysis; 2. To continuously incorporate the newest array products and analysis tools into the menu of services provided; 3. To educate and train users in applications, strengths, and limitations of microarray technology; 4. To assist users in experimental design, sample processing, and data analysis; 5. To provide logistic support for project development, grant applications, and manuscript preparations applying microarray technology. The Core presented here shall ensure that JGBCC investigators, especially clinical faculty and research groups, will be able to efficiently adopt this novel state-of-the-art technology for their research, clinical, and investigative goals using in vitro, clinical specimen, or animal model materials (using their approval protocols). It will be crucial for these investigators to have affordable access and expert support to the most advanced and sophisticated molecular technologies as part of the institutional resources provided to them.