COBRE: LOUISVILLE RES FOUND INC: CORE B: MICROARRAY FACILITY

COBRE：LOUISVILLE RES FOUND INC：核心 B：微阵列设施

• 批准号: 7382007
• 负责人: Wolfgang Zacharias
• 金额: $ 13.56万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7382007
• 关键词: COBRE; LOUISVILLE; RES; FOUND; INC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Microsequence Array Core Facility is located in the Baxter II Biomedical Research Building, one block from the Brown Cancer Center. This facility contains an Affymetrix Genechip instrument which is available to all MT-COBRE members. Plans are underway for the development of a tissue array component of this facility as well. The Microsequence Array Facility is under the direction of Dr. Wolfgang Zacharias and has been fully operational for the past three and one-half years. It contains a full-time staff member, Sabine Waigel, who provides technical assistance in sample processing, hybridization and data analysis. The Microsequence Array Core has a close relationship with the Laser Capture Microscopy facility in the Department of Pathology and a newly established Tumor Bank in the Brown Cancer Center. This allows the preparation of samples with known pathologic characteristics and appropriate control specimens. Dr. Zacharias is available for assistance with experimental design and data analysis. In March 2005, the new high-resolution Affymetrix Scanner 3000 upgrade was purchased and installed. This upgrade now allows us to use the newest generation of high-density whole genome v2.0 arrays for human or mouse for all COBRE investigators. Also, several software/data analysis workshops were organized and held on campus for training of microarray technology user

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。微序列阵列核心设施位于Baxter II生物医学研究大楼，距离布朗癌症中心一个街区。该设施包含一台Affymetrix基因芯片仪器，可供所有MT-Cobre成员使用。开发该设施的组织阵列部件的计划也在进行中。微序列阵列设施由沃尔夫冈·扎卡里亚斯博士领导，在过去三年半的时间里全面投入运作。它包括一名全职工作人员Sabine Waigel，他在样品处理、杂交和数据分析方面提供技术援助。微序列阵列核心与病理科的激光捕获显微镜设备和布朗癌症中心新建立的肿瘤库有着密切的关系。这使得制备具有已知病理特征的样本和适当的对照样本成为可能。Zacharias博士可以协助进行实验设计和数据分析。2005年3月，购买并安装了新的高分辨率Affymetrix扫描仪3000升级版。这一升级现在允许我们为所有Cobre研究人员使用最新一代高密度全基因组v2.0阵列，用于人类或小鼠。此外，还在校园内组织和举办了几个软件/数据分析讲习班，以培训微阵列技术用户