SOFTWARE FOR MASS SPECTRAL DATA CONVERSION / AUTOMATIC PROTEOMIC ANALYSIS

质谱数据转换/自动蛋白质组分析软件

• 批准号: 7182161
• 负责人: Catherine E. Costello
• 金额: $ 0.28万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7182161
• 关键词: biomedical equipment resource; biomedical resource; mass spectrometry; method development

DESCRIPTION: MALDI mass spectrometry has become one of the two important methods driving the application of mass spectrometry in biochemistry. A problem that stands in the way of broad application is the extensive metastable-ion decay exhibited by some molecules. This is a particular problem when a class of molecules has one or more labile groups that undergo reactions with significantly lower fragmentation barriers than those for analytically useful fragmentations. An example is the loss of water, for which there is little information content. The problem is made worse when a mixture is encountered. The composite fragmentation is difficult, if not impossible, to assign. A promising development to overcome this limitation is to initiate the desorption process in the presence of a bath gas. This idea will be pursued for the analysis of samples containing oligosaccharides, glyco and phosphopeptides, sialylated glycolipids, sulfated oligosaccharides, and peptides containing oxidized cysteine residues. Many of these samples are of interest to collaborators of the BU research resource.

摘要：MALDI质谱法已成为推动质谱法在生物化学领域应用的两种重要方法之一。阻碍广泛应用的一个问题是某些分子所表现出的广泛的亚稳离子衰变。当一类分子具有一个或多个不稳定基团时，这是一个特殊的问题，这些不稳定基团的分裂障碍比分析有用的分裂障碍明显低得多。一个例子是水的损失，其中几乎没有信息内容。当遇到混合物时，问题变得更糟。复合碎片很难（如果不是不可能的话）分配。克服这一限制的一个有希望的发展是在有浴气的情况下启动解吸过程。这个想法将被用于分析含有低聚糖、糖和磷酸肽、唾液化糖脂、硫酸化低聚糖和含有氧化半胱氨酸残基的肽的样品。这些样本中的许多都是波士顿大学研究资源的合作者感兴趣的。