DAKO ACIS II Image Analysis System for the University of South Carolina

南卡罗来纳大学的 DAKO ACIS II 图像分析系统

• 批准号: 7215064
• 负责人: Robert L Price
• 金额: $ 17.89万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7215064
• 关键词: American Cancer Society; Area; Cancer Center; Cardiovascular system; Cellular biology; Colon Carcinoma; Core Facility; Data; Development; Disease; Ensure; Environment; Funding; Grant; Histology; Housing; Image Analysis; Immunohistochemistry; Laboratories; Malignant Neoplasms; Minor; Neurosciences; North America; Numbers; Operative Surgical Procedures; Productivity; Purpose; Request for Proposals; Research; Research Personnel; Resources; Sampling; South Carolina; Staining method; Stains; Standardization; Standards of Weights and Measures; System; Travel; United States National Institutes of Health; Universities; anticancer research; cellular imaging; day; instrument; instrumentation; medical schools

DESCRIPTION (provided by applicant): DAKO Chromavision ACIS II Image Analysis System This proposal requests $175,850 to purchase a DAKO ACIS II image analysis system that will be housed in the Instrumentation Resource Facility (IRF) of the University of South Carolina School of Medicine and South Carolina Cancer Center (SCCC). Major users of the system will be 13 NIH funded researchers associated with the School of Medicine, the SCCC and the University of South Carolina Center for Colon Cancer Research (CCCR). Two other minor users of the system are young investigators that have American Cancer Society and/or NIH funding. The system will be used in a variety of research areas including cardiovascular development and disease, neurosciences and cancer. Collectively, the Users group holds 22 NIH grants. The DAKO ACIS II cell imaging system was selected as the instrument of choice after obtaining demonstrations on available systems at several meetings including FASEB, Cell Biology and the American Cancer Society meetings. In addition, Ms. Valerie Kennedy, the technologist who will be responsible for the day-to-day operation of the system, has traveled to the offices of DAKO North America, Inc. in Carpinteria, CA to analyze several samples relevant to the research of the Major Users. The purpose of this trip was to ensure the ACIS II system is capable of performing the image analysis tasks needed by our research group. The primary purpose for acquiring the system will be to automate our analyses of a number of standard immunohistochemistry (IHC) stains that are performed within the laboratories of the investigators participating in this proposal. This will significantly increase the acquisition and standardization of data from a variety of IHC stains produced for these investigators by the Histology Core in the IRF. Examples of the detailed analyses the ACIS II is capable of and how the automated features of the system will enhance the research productivity and competitiveness of each investigator is presented below in the Research Descriptions provided by the Major Users in this proposal. Acquisition of this system and housing it in an established core facility will allow access to it by a large number of investigators and will significantly enhance the research environment at the University of South Carolina.

描述（由申请人提供）：DAKO Chromavision ACIS II 图像分析系统 该提案要求花费 175,850 美元购买 DAKO ACIS II 图像分析系统，该系统将安装在南卡罗来纳大学医学院和南卡罗来纳癌症中心 (SCCC) 的仪器资源设施 (IRF) 中。该系统的主要用户将是 13 名 NIH 资助的研究人员，这些研究人员与医学院、SCCC 和南卡罗来纳大学结肠癌研究中心 (CCCR) 相关。该系统的另外两个小用户是获得美国癌症协会和/或美国国立卫生研究院资助的年轻研究人员。该系统将用于多种研究领域，包括心血管发育和疾病、神经科学和癌症。用户组总共拥有 22 项 NIH 拨款。在 FASEB、细胞生物学和美国癌症协会会议等多次会议上获得可用系统演示后，DAKO ACIS II 细胞成像系统被选为首选仪器。此外，负责系统日常运行的技术专家 Valerie Kennedy 女士已前往位于加利福尼亚州卡宾特里亚的 DAKO North America, Inc. 办公室，分析与主要用户研究相关的多个样本。此行的目的是确保ACIS II系统能够执行我们课题组所需的图像分析任务。获取该系统的主要目的是自动分析许多标准免疫组织化学 (IHC) 染色剂，这些染色剂是在参与本提案的研究人员的实验室内进行的。这将显着增加 IRF 组织学核心为这些研究人员生成的各种 IHC 染色数据的采集和标准化。以下主要用户在本提案中提供的研究描述中介绍了 ACIS II 能够进行的详细分析示例，以及系统的自动化功能将如何提高每个研究者的研究生产力和竞争力。购买该系统并将其安置在已建立的核心设施中将使大量研究人员能够使用该系统，并将显着改善南卡罗来纳大学的研究环境。