DAKO ACIS II Image Analysis System for the University of South Carolina

描述（由申请人提供）：DAKO Chromavision ACIS II图像分析系统该提案要求175,850美元用于购买DAKO ACIS II图像分析系统，该系统将安置在南卡罗来纳大学医学院和南卡罗来纳癌症中心（SCCC）的仪器资源设施（IRF）中。该系统的主要用户将是13名NIH资助的研究人员，他们与医学院、SCCC和南卡罗来纳大学结肠癌研究中心（CCCR）有关。该系统的另外两个次要用户是获得美国癌症协会和/或NIH资助的年轻研究人员。该系统将用于各种研究领域，包括心血管发育和疾病、神经科学和癌症。总的来说，用户组拥有22个NIH拨款。DAKO ACIS II细胞成像系统在FASEB、细胞生物学和美国癌症协会等会议上获得了可用系统的演示后，被选为首选仪器。此外，负责该系统日常操作的技术专家Valerie Kennedy女士已前往位于加州卡品特里亚的DAKO北美公司的办公室，分析与主要用户研究有关的几个样本。这次旅行的目的是确保ACIS II系统能够执行我们课题组所需的图像分析任务。获得该系统的主要目的是自动化我们在参与本提案的研究人员的实验室中进行的许多标准免疫组织化学（IHC）染色的分析。这将显著增加IRF中组织学核心为这些研究者制作的各种免疫组化染色数据的获取和标准化。ACIS II能够进行详细分析的例子，以及该系统的自动化特性将如何提高每位研究者的研究效率和竞争力，将在本提案中主要用户提供的研究描述中给出。购买该系统并将其安置在一个已建立的核心设施中，将允许大量研究人员使用它，并将大大改善南卡罗来纳大学的研究环境。

DESCRIPTION (provided by applicant): DAKO Chromavision ACIS II Image Analysis System This proposal requests $175,850 to purchase a DAKO ACIS II image analysis system that will be housed in the Instrumentation Resource Facility (IRF) of the University of South Carolina School of Medicine and South Carolina Cancer Center (SCCC). Major users of the system will be 13 NIH funded researchers associated with the School of Medicine, the SCCC and the University of South Carolina Center for Colon Cancer Research (CCCR). Two other minor users of the system are young investigators that have American Cancer Society and/or NIH funding. The system will be used in a variety of research areas including cardiovascular development and disease, neurosciences and cancer. Collectively, the Users group holds 22 NIH grants. The DAKO ACIS II cell imaging system was selected as the instrument of choice after obtaining demonstrations on available systems at several meetings including FASEB, Cell Biology and the American Cancer Society meetings. In addition, Ms. Valerie Kennedy, the technologist who will be responsible for the day-to-day operation of the system, has traveled to the offices of DAKO North America, Inc. in Carpinteria, CA to analyze several samples relevant to the research of the Major Users. The purpose of this trip was to ensure the ACIS II system is capable of performing the image analysis tasks needed by our research group. The primary purpose for acquiring the system will be to automate our analyses of a number of standard immunohistochemistry (IHC) stains that are performed within the laboratories of the investigators participating in this proposal. This will significantly increase the acquisition and standardization of data from a variety of IHC stains produced for these investigators by the Histology Core in the IRF. Examples of the detailed analyses the ACIS II is capable of and how the automated features of the system will enhance the research productivity and competitiveness of each investigator is presented below in the Research Descriptions provided by the Major Users in this proposal. Acquisition of this system and housing it in an established core facility will allow access to it by a large number of investigators and will significantly enhance the research environment at the University of South Carolina.