DAKO ACIS II Image Analysis System for the University of South Carolina

描述(由申请者提供)：DAKO Chromavision ACIS II图像分析系统这项计划申请175,850美元购买一个DAKO ACIS II图像分析系统，该系统将安装在南卡罗来纳大学医学院和南卡罗来纳癌症中心(SCCC)的仪器资源设施(IRF)中。该系统的主要用户将是NIH资助的13名研究人员，他们与医学院、SCCC和南卡罗来纳大学结肠癌研究中心(CCCR)有关。该系统的另外两个较小的用户是拥有美国癌症协会和/或NIH资金的年轻研究人员。该系统将用于各种研究领域，包括心血管发展和疾病、神经科学和癌症。总体而言，用户组拥有22项NIH拨款。DAKO ACIS II细胞成像系统在FASE B、细胞生物学和美国癌症学会会议等几次会议上获得了关于可用系统的演示后，被选为首选仪器。此外，将负责该系统日常运作的技术专家瓦莱丽·肯尼迪女士已前往达科北美公司在加利福尼亚州卡平特里亚的办事处，分析与主要用户研究有关的几个样品。此行的目的是确保ACIS II系统能够执行我们研究小组所需的图像分析任务。获得该系统的主要目的将是自动化我们对一些标准免疫组织化学(IHC)染色的分析，这些染色是在参与这项提议的研究人员的实验室内进行的。这将大大增加IRF组织学核心为这些研究人员提供的各种IHC染色数据的获取和标准化。ACIS II能够进行的详细分析的例子，以及该系统的自动化特征将如何提高每个研究人员的研究生产力和竞争力的例子，在本建议书主要用户提供的研究说明中如下所示。购买这一系统并将其存放在已建立的核心设施中，将使大量调查人员能够使用该系统，并将大大改善南卡罗来纳大学的研究环境。

DESCRIPTION (provided by applicant): DAKO Chromavision ACIS II Image Analysis System This proposal requests $175,850 to purchase a DAKO ACIS II image analysis system that will be housed in the Instrumentation Resource Facility (IRF) of the University of South Carolina School of Medicine and South Carolina Cancer Center (SCCC). Major users of the system will be 13 NIH funded researchers associated with the School of Medicine, the SCCC and the University of South Carolina Center for Colon Cancer Research (CCCR). Two other minor users of the system are young investigators that have American Cancer Society and/or NIH funding. The system will be used in a variety of research areas including cardiovascular development and disease, neurosciences and cancer. Collectively, the Users group holds 22 NIH grants. The DAKO ACIS II cell imaging system was selected as the instrument of choice after obtaining demonstrations on available systems at several meetings including FASEB, Cell Biology and the American Cancer Society meetings. In addition, Ms. Valerie Kennedy, the technologist who will be responsible for the day-to-day operation of the system, has traveled to the offices of DAKO North America, Inc. in Carpinteria, CA to analyze several samples relevant to the research of the Major Users. The purpose of this trip was to ensure the ACIS II system is capable of performing the image analysis tasks needed by our research group. The primary purpose for acquiring the system will be to automate our analyses of a number of standard immunohistochemistry (IHC) stains that are performed within the laboratories of the investigators participating in this proposal. This will significantly increase the acquisition and standardization of data from a variety of IHC stains produced for these investigators by the Histology Core in the IRF. Examples of the detailed analyses the ACIS II is capable of and how the automated features of the system will enhance the research productivity and competitiveness of each investigator is presented below in the Research Descriptions provided by the Major Users in this proposal. Acquisition of this system and housing it in an established core facility will allow access to it by a large number of investigators and will significantly enhance the research environment at the University of South Carolina.