BD Biosciences LSR II FACS for the University of South Carolina

描述（由申请人提供）：本提案要求277,596美元用于购买BD Biosciences LSR II FACS系统。该系统将被安置在南卡罗来纳大学医学院和结肠癌研究中心的仪器资源设施（IRF）中。IRF是一个已建立的核心生物技术设施，为南卡罗来纳大学以及南卡罗来纳州的其他几所本科和研究生机构提供服务。南卡罗来纳大学唯一可用的核心设施FACS系统是15年前的Beckman Coulter epic XL，它在激光，荧光激发和发射，信号检测和数据分析方面非常过时。值得注意的是，该提案的两个主要用户是大型中心拨款的pi，一个是结肠癌，一个是自身免疫性疾病和炎症。该系统的主要用户将是7名NIH资助的研究人员以及一名初级研究员，IRF的研究助理教授Udai Singh博士。除了两项中心拨款外，该小组还担任11项R01拨款的pi和一些其他NIH拨款的co - i。该系统将用于多种研究领域，包括炎症、免疫学、癌症、艾滋病、心血管发育和疾病。正如提案中详述的那样，南方大学的生物医学研究环境正在迅速扩大，研究界没有能够检测四种以上颜色的最先进的FACS系统。这对参与该提案的研究人员的研究项目以及南卡罗来纳大学招募其他有前途的科学家产生了不利影响。购买该系统并将其安置在一个已建立的核心设施中，将使大量研究人员能够使用该系统，并将大大改善南卡罗来纳大学和南卡罗来纳州的研究环境。公共卫生相关性：细胞分选在许多涉及炎症的疾病（如癌症、HIV和心血管肥大）的研究中是必不可少的。使用高分辨率、多色、高通量仪器（如BD Biosciences的LSR II分选机），最容易进行多种细胞类型的免疫学研究，以及区分细胞类型及其各种生物标志物的能力。

DESCRIPTION (provided by applicant): This proposal requests $277,596 to purchase a BD Biosciences LSR II FACS system. The system will be housed in the Instrumentation Resource Facility (IRF) of the University of South Carolina School of Medicine and Center for Colon Cancer Research. The IRF is an established core biotechnology facility serving the University of South Carolina as well as several other undergraduate and graduate institutions in the State of South Carolina. The only core facility FACS system available at the University of South Carolina is a 15 year old Beckman Coulter Epics XL, which is very outdated with regard to lasers, fluorochrome excitation and emission, signal detection, and data analysis. Notably, two of the Major Users on this proposal are PIs of large Center grants, one on colon cancer and one on autoimmune diseases and inflammation. Major users of the system will be 7 NIH funded researchers as well as a Junior Investigator, Dr. Udai Singh who is a Research Assistant Professor in the IRF. In addition to the two Center Grants the group serves as PIs of 11 R01 grants and Co-Is on a number of other NIH grants. The system will be used in a variety of research areas including inflammation, immunology, cancer, HIV-AIDS and cardiovascular development and disease. As detailed in the proposal the biomedical research environment at the University of South is rapidly expanding and no state of the art FACS systems capable of detecting more than four colors is available to the research community. This has adversely affected the research programs of the investigators participating in this proposal as well as the recruitment to the University of South Carolina of other promising scientists. Acquisition of this system and housing it in an established core facility will allow access to it by a large number of investigators and will significantly enhance the research environment at the University of South Carolina and in the State of South Carolina. Public Health Relevance: Cell sorting is essential in the study of many diseases that involve inflammation such as cancer, HIV, and cardiovascular hypertrophy. The immunology of the multiple cell types present and the ability to distinguish the types of cells present and their various biomarkers is most readily performed with high resolution, multi-color, high throughput instrumentation such as the requested BD Biosciences LSR II sorter.