BD Biosciences LSR II FACS for the University of South Carolina

南卡罗来纳大学的 BD Biosciences LSR II FACS

• 批准号: 7594884
• 负责人: Robert L Price
• 金额: $ 27.8万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-21 至 2010-05-20
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7594884
• 关键词: 15 year old; Affect; Area; Arts; Autoimmune Diseases; Biological Markers; Biomedical Research; Biotechnology; Cancer Immunology Science; Cardiovascular system; Cell Separation; Colon Carcinoma; Color; Communities; Core Facility; Data Analyses; Detection; Development; Disease; Environment; Fluorochrome; Funding; Grant; HIV; Housing; Hypertrophy; Immunology; Inflammation; Institution; Lasers; Malignant Neoplasms; Request for Proposals; Research; Research Personnel; Resolution; Resources; Scientist; Signal Transduction; South Carolina; System; United States National Institutes of Health; Universities; anticancer research; cell type; instrumentation; medical schools; professor; programs; public health relevance

DESCRIPTION (provided by applicant): This proposal requests $277,596 to purchase a BD Biosciences LSR II FACS system. The system will be housed in the Instrumentation Resource Facility (IRF) of the University of South Carolina School of Medicine and Center for Colon Cancer Research. The IRF is an established core biotechnology facility serving the University of South Carolina as well as several other undergraduate and graduate institutions in the State of South Carolina. The only core facility FACS system available at the University of South Carolina is a 15 year old Beckman Coulter Epics XL, which is very outdated with regard to lasers, fluorochrome excitation and emission, signal detection, and data analysis. Notably, two of the Major Users on this proposal are PIs of large Center grants, one on colon cancer and one on autoimmune diseases and inflammation. Major users of the system will be 7 NIH funded researchers as well as a Junior Investigator, Dr. Udai Singh who is a Research Assistant Professor in the IRF. In addition to the two Center Grants the group serves as PIs of 11 R01 grants and Co-Is on a number of other NIH grants. The system will be used in a variety of research areas including inflammation, immunology, cancer, HIV-AIDS and cardiovascular development and disease. As detailed in the proposal the biomedical research environment at the University of South is rapidly expanding and no state of the art FACS systems capable of detecting more than four colors is available to the research community. This has adversely affected the research programs of the investigators participating in this proposal as well as the recruitment to the University of South Carolina of other promising scientists. Acquisition of this system and housing it in an established core facility will allow access to it by a large number of investigators and will significantly enhance the research environment at the University of South Carolina and in the State of South Carolina. Public Health Relevance: Cell sorting is essential in the study of many diseases that involve inflammation such as cancer, HIV, and cardiovascular hypertrophy. The immunology of the multiple cell types present and the ability to distinguish the types of cells present and their various biomarkers is most readily performed with high resolution, multi-color, high throughput instrumentation such as the requested BD Biosciences LSR II sorter.

描述(由申请人提供)：这份建议书要求277,596美元购买BD Biosciences LSR II FACS系统。该系统将安装在南卡罗来纳大学医学院和结肠癌研究中心的仪器资源设施(IRF)中。研究所是南卡罗来纳大学以及南卡罗来纳州其他几个本科生和研究生机构的既定核心生物技术机构。南卡罗来纳大学唯一可用的核心设备FACS系统是一台已有15年历史的Beckman Coulter EPICS XL，在激光、荧光激发和发射、信号检测和数据分析方面非常过时。值得注意的是，这项提案的两个主要使用者是大笔中心赠款的个人投资者，一个是结肠癌，另一个是自身免疫性疾病和炎症。该系统的主要用户将是7名NIH资助的研究人员和一名初级研究员，他是IRF的研究助理教授Udai Singh博士。除了两个中心拨款外，该组织还担任11个R01拨款的私人投资机构和其他一些NIH赠款的联合资助机构。该系统将用于各种研究领域，包括炎症、免疫学、癌症、艾滋病毒-艾滋病以及心血管发展和疾病。正如提案中详细描述的那样，南方大学的生物医学研究环境正在迅速扩大，研究界还没有能够检测四种以上颜色的最先进的FACS系统。这对参与这项提议的研究人员的研究计划以及南卡罗来纳大学招募其他有前途的科学家的工作产生了不利影响。购买这一系统并将其存放在一个既定的核心设施中，将使大量调查人员能够使用该系统，并将大大改善南卡罗来纳大学和南卡罗来纳州的研究环境。公共卫生相关性：细胞分选在许多涉及炎症的疾病的研究中是必不可少的，例如癌症、艾滋病毒和心血管肥大。目前存在的多种细胞类型的免疫学以及区分存在的细胞类型和它们的各种生物标志物的能力最容易利用高分辨率、多颜色、高通量的仪器来执行，例如所要求的BD Bioscience LSR II分选仪。