LASER CONFOCAL MICROSCOPE

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：共聚焦显微镜已经成为满足生物医学研究人员需求的不可或缺的工具。在爱达荷大学，这一点在我们现有的共焦显微镜BioRad 1024的使用增加中可见一斑。虽然BioRad在1994年购买时就足够了，但更新的仪器具有更好的光谱分离，使研究人员能够提出通常几年前共焦仪器之外的问题。美国国立卫生研究院资助的爱达荷大学的生物医学研究在这十年里有了显著的增长。除了几项新的R01拨款外，由于大学位于符合IDEA资格的州，该大学还获得了两笔科布雷中心拨款和NCRR的INBRE基础设施项目。这项提议是购买一台徕卡TCS SP2共聚焦显微镜，以支持七名由NIH资助的研究人员的研究，其中四人获得R01奖，第五人将于2005年7月开始研究。他们的专业领域从发育神经生物学到微生物和生物膜研究。此外，也有联邦资助项目的五个小用户将利用该工具作为未来NIH资助的基础。我们已经获得了新的徕卡和蔡司显微镜以及我们自己的BioRad的初步数据。这些初步结果包括在提案中。我们选择了基本的徕卡共焦，因为它将以适中的价格满足我们目前的需求。此外，它显然更容易培训新用户，因为它由一个直立显微镜组成，而且可以在未来的补充资金下很容易地升级到双光子能力。我们现有的共焦核心实验室将于2005年夏天升级，毗邻一个组织培养实验室和湿实验室，以促进生物膜和微生物研究。该业务计划包括一个内部咨询小组和大学对服务合同和高级专家技术人员的支持。新的徕卡共焦显微镜将满足我们的研究人员在未来多年回答各自领域的下一组问题的关键需求。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Confocal microscopy has become an indispensable tool in meeting the needs of biomedical researchers. This is evident at the University of Idaho in the increased use of our existing confocal microscope, a BioRad 1024. While the BioRad was adequate when purchased in 1994, newer instruments with better spectral separation enable researchers to ask questions beyond the confocal instruments often years ago. NIH- funded biomedical research at the University of Idaho has grown significantly in these ten years. In addition to several new R01 grants the University, by virtue of its location in an IDeA-eligible state, is host to two COBRE Center grants and the INBRE infrastructure program from the NCRR. This proposal is to purchase a Leica TCS SP2 confocal microscope to support the research of seven NIH-funded investigators, four of whom have R01 awards, and a fifth will begin in July 2005. Their areas of expertise range from developmental neurobiology to microbial and biofilm research. In addition five minor users, who also have federally funded projects, will make use of the instrument as the basis for future NIH funding. We have acquired preliminary data on new Leica and Zeiss microscopes, as well as our own BioRad. These preliminary results are included in the proposal. We have selected the basic Leica confocal because it will meet our current needs at modest cost. Moreover, it is demonstrably easier for training new users, as it consists of an upright microscope, and can be readily upgraded to two-photon capability with future supplementary funding. Our existing confocal core lab will be upgraded in summer 2005 with an adjoining tissue culture lab and wet lab to facilitate biofilm and microbial studies. The business plan includes an internal advisory group and university support for a service contract and senior expert technician. The new Leica confocal microscope will meet a critical need of our investigators to answer the next set of questions in each of their fields for many years to come.