BEHAVIOR AND BRAIN IMAGING IN BABOONS

狒狒的行为和大脑成像

• 批准号: 7349760
• 负责人: ROBERT E SHADE
• 金额: $ 5.66万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349760
• 关键词: BEHAVIOR; BRAIN; IMAGING; BABOONS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Very little is known in regard to the neurohumoral factors that regulative ingestive behaviors in primate species. In earlier work, we showed that increased salt intake behavior produced by salt depletion in the baboon requires functional brain angiotensin mechanism. However, components of the hypothalamic-pituitary-adrenal axis that are potent stimuli for salt and water intake in mice, rats, rabbits and ungulates do not have any effect on ingestive behaviors in baboons. The objective of this study is to develop a baboon preparation that will permit administration of neurohumoral factors to brain structures so we can observe activation of brain centers using fMRI technology. The initial steps require development of a non-ferrous metal cannula, system for administration of hormonal and neurotransmitter agonists and antagonists into brain CSF while collecting fMRI images for brain activation observations. An intravenous anesthetic protocol ahs been developed that permits sedation of baboons for periods of up to 3 hours wile collecting brain images. Using this procedure we have replicated in baboons a recent study we conducted in humans in which intravenous saline infusion was used to stimulate thirst while collecting PET images of brain activation. The areas of the baboon brain activated by hypertonic saline were identical to the brain centers activated in the human studies. We will explore several approaches for a non-metallic cannula system for implanting in the baboon lateral brain ventricle. The system will enable the collection of PET images of brain activation responses to stimuli applied directly to the brain.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。关于调节灵长类动物摄食行为的神经体液因子知之甚少。在早期的研究中，我们发现狒狒因盐缺乏而增加的盐摄入行为需要功能性脑血管紧张素机制。然而，下丘脑-垂体-肾上腺轴的组成部分，是强有力的刺激盐和水的摄入量在小鼠，大鼠，兔和有蹄类动物没有任何影响狒狒的摄食行为。本研究的目的是开发一种狒狒制剂，将允许管理的神经体液因子的大脑结构，所以我们可以观察激活的大脑中心使用功能磁共振成像技术。最初的步骤需要开发一种有色金属套管，用于将激素和神经递质激动剂和拮抗剂施用到脑CSF中的系统，同时收集用于脑激活观察的fMRI图像。静脉麻醉方案已经开发，允许狒狒镇静长达3小时，同时收集脑图像。使用这个程序，我们在狒狒身上复制了我们最近在人类身上进行的一项研究，在这项研究中，静脉注射盐水来刺激口渴，同时收集大脑激活的PET图像。高渗盐水激活的狒狒大脑区域与人类研究中激活的大脑中心相同。我们将探讨几种方法的非金属套管系统植入狒狒侧脑室。该系统将能够收集对直接施加到大脑的刺激的大脑激活反应的PET图像。