MALDI & ESI & LC ESI QQTOF MS TRAINING

马尔迪

• 批准号: 7369237
• 负责人: Catherine E. Costello
• 金额: $ 0.67万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369237
• 关键词: MALDI; ESI; LC; QQTOF; MS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our laboratory makes extensive use of the Applied Biosystems API QSTAR(tm) Pulsar i QqTOF mass spectrometer for the characterization of complex biological samples obtained from collaborative research projects. During the current reporting year, several staff members have been provided training on the design and use of the QSTAR for the analysis of samples via ESI-MS/MS, MALDI-MS/MS and LC-ESI-MS/MS. They have been trained in the use of batch parameter files, processing scripts and automated IDA MS/MS functions that have been optimized for typical protein digests. LC instruction has been provided and includes theory and application of column packing methodology, LC separation parameters and ESI interfaces.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。我们的实验室广泛使用Applied Biosystems API QSTAR（tm）Pulsar i QqTOF质谱仪，用于表征从合作研究项目中获得的复杂生物样品。在本报告年度，几名工作人员接受了通过ESI-MS/MS、MALDI-MS/MS和LC-ESI-MS/MS分析样品的QSTAR设计和使用培训。他们接受了使用批参数文件、处理脚本和针对典型蛋白质分析优化的自动IDA MS/MS功能的培训。提供了液相色谱教学，包括柱填充方法学的理论和应用，液相色谱分离参数和ESI接口。