EFFECT OF SYSTEMIC HYPOXIA ON MSNA & ITS RELATIONSHIP TO INTERSTITIAL ADENINE NU

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。在全身性缺氧时，交感神经活动增加，骨骼肌间质腺苷和去甲肾上腺素增加。目前尚不清楚间质腺苷的增加是来自血管内皮、骨骼肌纤维还是来自细胞外ATP的转化。在这项研究中，我们建议在三种截然不同的交感神经兴奋干预中，通过肌内微透析探针同时测量去甲肾上腺素和腺嘌呤核苷酸（ATP， ADP和AMP），我们将获得区分独特血管对缺氧反应的必要条件。将使用的三种不同的15分钟刺激包括：1)全身缺氧；2)静脉滴注硝普钠；3)下半身负压。将对16名志愿者进行研究。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During systemic hypoxia, sympathetic nerve activity is increased accompanied by increases in skeletal muscle interstitial adenosine and norepinephrine. It is not known whether the increases in interstitial adenosine come from vascular endothelium, from skeletal muscle fiber or from extra cellular conversion of ATP. In this study, we propose that through simultaneous measurement of norepinephrine and adenine nucleotides (ATP, ADP, and AMP) from intramuscular microdialysis probes during three distinctly different sypatho excitatory interventions we will obtain the necessary to differentiate unique vascular responses to hypoxia. The three different 15 minute stimuli that will be used include: 1) systemic hypoxia; 2) intravenous infusion of sodium nitroprusside; and 3) lower body negative pressure. 16 volunteers will be studied.