IMPROVED MASS MEASUREMENT IN FTMS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。准确测量质量的能力是傅里叶变换质谱法（FTMS）的重要特性。与质谱中的其他仪器方法不同，FTMS仍然为提高质量测量精度（MMA）提供了重要的机会，使其成为一个研究领域。虽然FTMS可以测量到9个有效数字的离子频率，但频率转换为m/z是困难的，因为频率取决于电场和离子阱中的空间电荷。我们正在研究填充电场（电补偿）和校正空间电荷效应的方法。我们还对质量校准与离子运动和空间电荷效应的基本原理之间的关系感兴趣，我们希望改进基本校准程序，特别是那些校正空间电荷效应的程序。基质辅助激光解吸电离（MALDI）和电喷雾电离（ESI）的出现为FTMS用于分析高性能生物聚合物打开了大门，包括蛋白质、寡脱氧核苷酸（odn）、低聚糖和合成聚合物。我们将在这些方面追求精确的质量测量和一些切实可行的方法来提高MMA。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The capability for accurate mass measurements is an important attribute of Fourier transform mass spectrometry (FTMS). Unlike other instrumental methods in mass spectrometry, FTMS still offers significant opportunities to improve mass measurement accuracy (MMA), making it an area of research. Although FTMS can measure ion frequencies to nine significant figures, the conversion of frequency to m/z is difficult because frequency depends on the electric field and the space charge in the ion trap. We are working on methods to shim the electric field (electrical compensation) and to correct for space charge effects. We are also interested in the relation of mass calibration and the fundamentals of ion motion and space-charge effects, and we wish to to improve the basic calibration procedure particularly those that correct for effects of space charge. The advent of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) have opened the door for FTMS to be used for analyzing at high performance biopolymers, including proteins, oligodeoxynucleotides (ODNs), oligosaccharides, and synthetic polymers. We will pursue accurate mass measurement in these areas and some practical ways to improve MMA.