IMPROVED MASS MEASUREMENT IN FTMS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 精确质量测量的能力是傅里叶变换质谱（FTMS）的重要属性。 与质谱中的其他仪器方法不同，FTMS仍然提供了提高质量测量准确度（MMA）的重要机会，使其成为一个研究领域。 虽然FTMS可以测量离子频率到九位有效数字，但将频率转换为m/z是困难的，因为频率取决于离子阱中的电场和空间电荷。 我们正在研究调整电场（电补偿）和校正空间电荷效应的方法。 我们也有兴趣在质量校准和离子运动和空间电荷效应的基本原理的关系，我们希望改进的基本校准程序，特别是那些校正的空间电荷的影响。 基质辅助激光解吸电离（MALDI）和电喷雾电离（ESI）的出现为FTMS用于分析高性能生物聚合物（包括蛋白质、寡脱氧核苷酸（ODN）、寡糖和合成聚合物）打开了大门。 我们将在这些领域追求精确的质量测量和一些实用的方法来提高MMA。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The capability for accurate mass measurements is an important attribute of Fourier transform mass spectrometry (FTMS). Unlike other instrumental methods in mass spectrometry, FTMS still offers significant opportunities to improve mass measurement accuracy (MMA), making it an area of research. Although FTMS can measure ion frequencies to nine significant figures, the conversion of frequency to m/z is difficult because frequency depends on the electric field and the space charge in the ion trap. We are working on methods to shim the electric field (electrical compensation) and to correct for space charge effects. We are also interested in the relation of mass calibration and the fundamentals of ion motion and space-charge effects, and we wish to to improve the basic calibration procedure particularly those that correct for effects of space charge. The advent of matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) have opened the door for FTMS to be used for analyzing at high performance biopolymers, including proteins, oligodeoxynucleotides (ODNs), oligosaccharides, and synthetic polymers. We will pursue accurate mass measurement in these areas and some practical ways to improve MMA.