CHARACTERIZATION OF YPKA PATHOGENICITY IN HOST CELLS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I plan to work with Ben Giepmans to characterize the pathogenicity of YpkA, a Yersinia effector protein. Currently, it is know that YpkA is responsible for the dissolution of the actin cytoskeleton once it has been introduced into host cells. The mechanism by which this occurs, however, is thus far unknown. We plan to transfect or microinject YpkA plasmids into host cells that stably express GFP-actin or potentially mCherry-actin. Through the use of confocal microscopy, we can then resolve more clearly the spatio-temporal distribution of the pathogenic protein as well as the resulting actin disruption.