CHARACTERIZATION OF YPKA PATHOGENICITY IN HOST CELLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我计划与Ben Giepmans合作，研究耶尔森菌效应蛋白YpkA的致病性。 目前，已知一旦YpkA被引入宿主细胞，其负责肌动蛋白细胞骨架的溶解。 然而，这种情况发生的机制迄今尚不清楚。 我们计划将YpkA质粒转染或显微注射到稳定表达GFP-肌动蛋白或潜在的mCherry-肌动蛋白的宿主细胞中。 通过使用共聚焦显微镜，我们可以更清楚地解析致病蛋白的时空分布以及由此产生的肌动蛋白破坏。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I plan to work with Ben Giepmans to characterize the pathogenicity of YpkA, a Yersinia effector protein. Currently, it is know that YpkA is responsible for the dissolution of the actin cytoskeleton once it has been introduced into host cells. The mechanism by which this occurs, however, is thus far unknown. We plan to transfect or microinject YpkA plasmids into host cells that stably express GFP-actin or potentially mCherry-actin. Through the use of confocal microscopy, we can then resolve more clearly the spatio-temporal distribution of the pathogenic protein as well as the resulting actin disruption.