MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

膜结合 RNA 病毒复制复合物

• 批准号: 7722401
• 负责人: PAUL G. AHLQUIST
• 金额: $ 1.17万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722401
• 关键词: Binding; Bromus; Cell membrane; Class; Complex; Computer Retrieval of Information on Scientific Projects Database; Cytoplasm; DNA Sequence Rearrangement; Drosophila genus; Electron Microscope; Family; Funding; Genetic; Grant; Housing; Human; Institution; Left; Membrane; Modeling; Mosaic Viruses; Nature; Outer Mitochondrial Membrane; RNA Viruses; RNA chemical synthesis; RNA replication; Relative (related person); Research; Research Personnel; Resources; Site; Source; Structure; United States National Institutes of Health; Vesicle; Viral; Virus; Virus Replication; member; pathogen; tomography; transmission process; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The three-dimensional nature of electron microscope tomography (EMT) overcomes many serious limitations of two-dimensional EM analysis to reveal many fundamental features not accessible from conventional transmission EM analyses. We employ EMT and the advanced model viruses, flock house virus (FHV) and brome mosaic virus (BMV), to understand the organization of the membrane-bounded, vesicle-like RNA replication complexes (spherules) induced by many important positive-strand RNA viruses. Positive-strand RNA viruses are the largest genetic class of viruses and include many serious human pathogens. RNA replication by positive-strand RNA viruses is universally associated with membranes and membrane rearrangements such as single or double membrane vesicles. However, in most cases the site(s) of RNA synthesis relative to these rearranged membranes and the topological relationships among membranes, vesicle interiors, lumenal compartments, and the cytoplasm are poorly defined. Recent results suggest that, in at least some cases, viral RNA replication occurs inside such vesicles. However, these findings leave many unresolved questions, in many ways due to limitations inherent to the two-dimensional analyses used. The best-studied member of the nodavirus family, FHV, replicates at the outer mitochondrial membranes of infected Drosophila cells where membrane-bound spherules form in the intermembrane space, similar to multi-vesicular structures observed for many positive-strand RNA viruses.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 电子显微镜断层扫描（EMT）的三维性质克服了二维EM分析的许多严重局限性，揭示了从常规传播EM分析中无法获得的许多基本特征。我们采用EMT和晚期模型病毒，羊群病毒（FHV）和Brome Mosaic病毒（BMV），以了解许多由许多重要的阳性RNA病毒引起的膜结合的膜状RNA复制复合复合物（Spherules（Spherules））的组织。阳性链RNA病毒是最大的遗传性病毒，包括许多严重的人类病原体。阳性RNA病毒的RNA复制与膜和膜重排（如单膜或双膜囊泡）普遍相关。但是，在大多数情况下，RNA合成的位点相对于这些重排的膜以及膜，囊泡内部，腔室和细胞质之间的拓扑关系的定义很差。最近的结果表明，至少在某些情况下，病毒RNA复制发生在此类囊泡内。但是，由于所使用的二维分析固有的局限性，这些发现在许多方面都留下了许多未解决的问题。 Nodavirus家族中最有研究的成员FHV在感染的果蝇细胞的外部线粒体膜上复制，其中在膜间空间中形成了膜结合的球形，类似于许多阳性RNA病毒观察到的多剂量结构。