CHARACTERIZATION OF BMV REPLICATION COMPLEX

雀麦花叶病毒（BMV）是一种甲病毒属病毒 是一种感染植物的（+）-链RNA病毒。 BMV RNA 复制完全发生在受感染细胞的细胞质中， 需要2种病毒编码的蛋白质和几种宿主因子。 研究 BMV和相关病毒的RNA复制表明， 与细胞质膜的联系， 可能涉及病毒诱导的胞质囊泡。 我们正在使用 电子显微镜进一步表征亚细胞 定位和组成这些专门的囊泡。 冷冻固定 技术将证明在这些研究中是有用的， 膜超微结构的保存。 我们希望能够描述 病毒诱导的囊泡在两个大麦原生质体，自然宿主 BMV，以及在酿酒酵母中， BMV复制的系统。

Brome mosaic virus (BMV) is a member of the alphavirus-like superfamily and is a plant-infecting, (+)-strand RNA virus. BMV RNA replication occurs entirely in the cytoplasm of infected cells and requires 2 virally-encoded proteins and several host factors. Studies of BMV and related viruses indicate that RNA replication occurs in association with cytoplasmic membranes and that specialized virus-induced cytoplasmic vesicles are likely involved. We are using electron microscopy to characterize further the subcellular localization and makeup of these specialized vesicles. Cryofixation techniques will prove useful in these studies to help in the preservation of membrane ultrastructure. We hope to characterize virus-induced vesicles in both barley protoplasts, the natural host of BMV, and also in Saccharomyces cerevisia, a genetically tractable system in which BMV replicates.