MEMBRANE BOUND RNA VIRUS REPLICATION COMPLEXES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 电子显微镜断层扫描揭示了诺达病毒RNA复制室的膜结构和拓扑结构。正链RNA病毒的一个普遍特征是宿主细胞内膜参与了RNA复制。与病毒RNA复制相关的细胞膜重排已在许多正链RNA病毒中观察到，包括甲病毒、小核糖核酸病毒、黄病毒等。在诺达病毒群屋病毒(FHV)中，病毒RNA的合成发生在线粒体膜上，并在线粒体膜内外之间形成40个60 nm的囊泡或球体。对于甲病毒超家族中的雀麦花叶病毒，已发现RNA复制发生在内质网膜上极其相似的球体内。为了更好地确定这种病毒RNA复制结构的结构和拓扑，我们用电子显微镜断层扫描技术研究了FHV感染的果蝇细胞中形成的球体，这使得细胞结构可以在三维上可视化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The use of electron microscope tomography revealed the membrane structure and topology of nodavirus RNA replication compartments. A universal feature of positive-strand RNA viruses is the involvement of host intracellular membranes in RNA replication. Intracellular membrane rearrangements associated with viral RNA replication have been observed for many positive-strand RNA viruses including alphaviruses, picornaviruses, flaviviruses, and others. In the nodavirus flock house virus (FHV), viral RNA synthesis occurs on mitochondrial membranes in association with formation of 40  60 nm vesicles or spherules between the inner and outer mitochondrial membranes. For brome mosaic virus in the alphavirus superfamily, RNA replication has been found to occur inside strikingly similar spherules on endoplasmic reticulum membranes. To better define the structure and topology of such viral RNA replication structures, we studied the spherules formed in FHV-infected Drosophila cells by electron microscope tomography, which permits the visualization of cellular structures in three dimensions.